Articles
THE USE OF NUCLEIC ACID PROBES FOR THE DETECTION OF PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNE DWARF VIRUS
Article number
309_8
Pages
79 – 84
Language
Abstract
Nucleic acid probes specific for Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV), respectively, were used to detect these viruses in peach.
The probes detected the viruses sensitively and unambiguously in both floral and leaf tissues.
It was possible to detect both viruses in leaf tissue and discriminate between healthy and virus-infected material throughout the growing season.
This is unlike the situation that exists when ELISA is used to assay for the presence of these viruses.
When using ELISA, PNRSV was detectable in peach tissues until stem elongation ceased but in subsequent assays it was impossible to discriminate unambiguously between samples from PNRSV-infected material and healthy material.
PDV was detectable by ELISA only for the very short period between bud swell and about 2 weeks after petal fall.
The ability of these probes to detect the viruses when ELISA was unsuccessful makes them eminently suitable for use when indexing material for virus-free programmes.
The probes detected the viruses sensitively and unambiguously in both floral and leaf tissues.
It was possible to detect both viruses in leaf tissue and discriminate between healthy and virus-infected material throughout the growing season.
This is unlike the situation that exists when ELISA is used to assay for the presence of these viruses.
When using ELISA, PNRSV was detectable in peach tissues until stem elongation ceased but in subsequent assays it was impossible to discriminate unambiguously between samples from PNRSV-infected material and healthy material.
PDV was detectable by ELISA only for the very short period between bud swell and about 2 weeks after petal fall.
The ability of these probes to detect the viruses when ELISA was unsuccessful makes them eminently suitable for use when indexing material for virus-free programmes.
Authors
S.W. Scott, V. Bowman-Vance, E. J. Bachman
Keywords
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