Articles
IN VITRO AND IN VIVO EXPRESSION OF PPV HELPER-COMPONENT GENE
Article number
309_28
Pages
197 – 202
Language
Abstract
In vitro translation experiments have been carried out with cRNAs obtained by transcription of cloned cDNAs representing the first two cistrons and the N-terminal part of the third cistron of plum pox virus (strain D): our results show that the polypeptide encoded by this genomic segment has proteolytic activity.
The protease activity was located to the C-terminal region of the polyprotein precursor corresponding to plum pox virus cistrons 1 and 2, like the helper-protease of the tobacco etch and tobacco vein mottling potyviruses.
The protease activity was located to the C-terminal region of the polyprotein precursor corresponding to plum pox virus cistrons 1 and 2, like the helper-protease of the tobacco etch and tobacco vein mottling potyviruses.
Authors
M. Ravelonandro, G. Tavert-Roudet, L. Garrigue, O. Peyruchaud, J. Dunez
Keywords
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