Articles
ARTIFICIAL SEEDS OF PEPPER SOMATIC EMBRYOS
Article number
412_11
Pages
106 – 110
Language
Abstract
Artificial seeds are a potential method for delivering somatic embryos to the field.
Mature somatic embryos were encapsulated in calcium alginate gel using different concentrations of sodium alginate (2–6%) and calcium chloride (25–100 mM) in order to determine the effect of rigidity of bead on the conversion frequency of somatic embryos.
MS medium supplemented with 3% sucrose was used for germination.
Variability was obtained between sodium alginate and calcium chloride concentration with respect to % germination.
Plant regeneration frequency decreased with an increase in sodium alginate concentration above 3%. 3% sodium alginate and 75 mM calcium chloride gave the best conversion and optimum bead rigidity and shape. In vitro and in vivo conversion of naked and encapsulated embryos were compared.
Mature somatic embryos were encapsulated in calcium alginate gel using different concentrations of sodium alginate (2–6%) and calcium chloride (25–100 mM) in order to determine the effect of rigidity of bead on the conversion frequency of somatic embryos.
MS medium supplemented with 3% sucrose was used for germination.
Variability was obtained between sodium alginate and calcium chloride concentration with respect to % germination.
Plant regeneration frequency decreased with an increase in sodium alginate concentration above 3%. 3% sodium alginate and 75 mM calcium chloride gave the best conversion and optimum bead rigidity and shape. In vitro and in vivo conversion of naked and encapsulated embryos were compared.
Authors
S. Büyükalaca, F. Mavituna
Keywords
Capsicum annuum, encapsulation, somatic embryogenesis
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