Articles
LARGE SCALE PRODUCTION OF PEPPER SOMATIC EMBRYOS USING BIOREACTORS
Article number
412_4
Pages
58 – 63
Language
Abstract
Bioreactors can be used for large scale production of somatic embryos effectively as labour saving system.
Two different types of bioreactor, an airlift and a magnetically stirred "hanging stirrer bar" bioreactor, each with 1.8 1 working volume, and different mixing/aeration rates were examined to determine a system capable of in vitro pepper somatic embryo production.
Production of pepper somatic embryos in a bioreactor was successful when a hanging stirrer bar bioreactor was used.
Mixing, aeration, inoculum type and level affect directly the somatic embryogenesis in a bioreactor.
The best results were obtained from hanging stirrer bar bioreactor at 0.45 vvm aeration and 80 rpm mixing rate in terms of percent embryogenesis.
These results cofirm that bioreactor culture offers promising aspects for large scale production.
Two different types of bioreactor, an airlift and a magnetically stirred "hanging stirrer bar" bioreactor, each with 1.8 1 working volume, and different mixing/aeration rates were examined to determine a system capable of in vitro pepper somatic embryo production.
Production of pepper somatic embryos in a bioreactor was successful when a hanging stirrer bar bioreactor was used.
Mixing, aeration, inoculum type and level affect directly the somatic embryogenesis in a bioreactor.
The best results were obtained from hanging stirrer bar bioreactor at 0.45 vvm aeration and 80 rpm mixing rate in terms of percent embryogenesis.
These results cofirm that bioreactor culture offers promising aspects for large scale production.
Authors
S. Büyükalaca, F. Mavituna
Keywords
Capsicum annum, liquid culture, scale-up, somatic embryogenesis
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