Articles
PRODUCTION OF PLANTS TOLERANT TO MULTIPLE VIRAL INFECTIONS BY GENETIC MANIPULATIONS
Article number
420_13
Pages
52 – 57
Language
Abstract
Disease resisitance is an important target for breeding ornamental plants as well as crops.
Technologies to make plants resistant against multiple virus infections are particularly interesting for us, because it is difficult to introduce several different viral genes for protection from those viral attacks.
We have developed a unique method to produce plants tolerant to multiple viral infections by using double stranded RNA specific ribonuclease (dsRNase) genes.
It has been demonstrated that a mutated transgene for pokeweed antiviral protein gives broad-spectrum virus resistance in transgenic tobacco and potato plants.
And it has been reported that the transgenic tobacco and potato plants expressing rat 2–5A synthetase showed tolerance to multiple viral infections.
It was deduced that the 2–5A/RNase L system was activated in the transgenic plants infected with viruses. we have also made transgenic tobacco expressing human 2–5A synthetase and found that some of the transgenic tobaccos expressed 2–5A synthetase activity.
But we could not find significant tolerance to TMV infection between the transgenic expressing the enzyme activity and non-transgenic tobacco plants.
We assayed endogenous ribonuclease L (RNase L) activity in the tobacco plant cells by use of tetramer 2–5A triphosphate molecules, we could not detect any 2–5A dependent ribonuclease activity.
Technologies to make plants resistant against multiple virus infections are particularly interesting for us, because it is difficult to introduce several different viral genes for protection from those viral attacks.
We have developed a unique method to produce plants tolerant to multiple viral infections by using double stranded RNA specific ribonuclease (dsRNase) genes.
It has been demonstrated that a mutated transgene for pokeweed antiviral protein gives broad-spectrum virus resistance in transgenic tobacco and potato plants.
And it has been reported that the transgenic tobacco and potato plants expressing rat 2–5A synthetase showed tolerance to multiple viral infections.
It was deduced that the 2–5A/RNase L system was activated in the transgenic plants infected with viruses. we have also made transgenic tobacco expressing human 2–5A synthetase and found that some of the transgenic tobaccos expressed 2–5A synthetase activity.
But we could not find significant tolerance to TMV infection between the transgenic expressing the enzyme activity and non-transgenic tobacco plants.
We assayed endogenous ribonuclease L (RNase L) activity in the tobacco plant cells by use of tetramer 2–5A triphosphate molecules, we could not detect any 2–5A dependent ribonuclease activity.
Feasibility of application of above methods on ornamental flower business will be discussed.
Authors
I. Ishida, T. Ogawa, M. Yoshioka, T. Hori, T. Ohtani
Keywords
Online Articles (41)