Articles
GENERATION OF NOVEL PATTERNS IN LISIANTHUS FLOWERS USING AN ANTISENSE CHALCONE SYNTHASE GENE
Article number
420_5
Pages
26 – 28
Language
Abstract
Antisense-mediated shutdown of flavonoid gene expression can produce patterns in some flowers (Van der Krol et al. 1988). We wish to determine whether an antisense chalcone synthase gene (CHS) is capable of producing patterns in lisianthus (Eustoma grandiflorum (Grise.)) flowers.
Transgenic lisianthus plants carrying a homologous antisense CHS gene were generated via Agrobacterium-mediated transformation using a purple flowering line ‘No. 54’ as the host plant.
Thirty five transgenic lines have flowered of which twenty have altered flower colour, ranging from small streaks of white through to completely white flowers.
The commercial cultivar Wakamurasaki (dark purple flowers) was also transformed with this construct.
Four out of five plants that have flowered show patterns in the flowers.
Transgenic lisianthus plants carrying a homologous antisense CHS gene were generated via Agrobacterium-mediated transformation using a purple flowering line ‘No. 54’ as the host plant.
Thirty five transgenic lines have flowered of which twenty have altered flower colour, ranging from small streaks of white through to completely white flowers.
The commercial cultivar Wakamurasaki (dark purple flowers) was also transformed with this construct.
Four out of five plants that have flowered show patterns in the flowers.
Authors
S. Deroles, M. Bradley, K. Davies, K. Schwinn, D. Manson
Keywords
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