Articles
SHOOT REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF CARNATION
Article number
420_24
Pages
92 – 94
Language
Abstract
Adventitious shoots were generated from leaf explants of carnation (Dianthus caryophyllus L.). Regeneration was only possible at the transition region from stem to leaf: the leaf base.
The position of leaves on the plant appeared to be important for subsequent regeneration and youngest leaves had the highest regeneration capacity.
The shoot regeneration procedure was efficient, 65 % of the explants showed regeneration.
For the development of an Agrobacterium-mediated transformation procedure, an intron containing
-glucuronidase (gus) gene was used to monitor the frequency of transformation events soon after infection of leaf explants.
The efficiency of gene transfer was dependent on the carnation genotype, explant age and cocultivation time.
After selection on a kanamycin-containing medium, transgenic shoots were generated among a relatively high number of untransformed shoots.
Transgenic plants were obtained from five cultivars after infection of leaf explants with the supervirulent Agrobacterium strain AGLO. Integration of the genes into the carnation genome was demonstrated by Southern blot hybridization.
Transformants were morphologically identical to untransformed plants.
Segregation of the genes occured in a Mendelian way.
The position of leaves on the plant appeared to be important for subsequent regeneration and youngest leaves had the highest regeneration capacity.
The shoot regeneration procedure was efficient, 65 % of the explants showed regeneration.
For the development of an Agrobacterium-mediated transformation procedure, an intron containing
-glucuronidase (gus) gene was used to monitor the frequency of transformation events soon after infection of leaf explants.The efficiency of gene transfer was dependent on the carnation genotype, explant age and cocultivation time.
After selection on a kanamycin-containing medium, transgenic shoots were generated among a relatively high number of untransformed shoots.
Transgenic plants were obtained from five cultivars after infection of leaf explants with the supervirulent Agrobacterium strain AGLO. Integration of the genes into the carnation genome was demonstrated by Southern blot hybridization.
Transformants were morphologically identical to untransformed plants.
Segregation of the genes occured in a Mendelian way.
Authors
A. Altvorst, T. Riksen, H. Koehorst, H. Dons
Keywords
Online Articles (41)