Articles
MICROPROPAGATION OF SOME SOUTH AFRICAN HYPOXIS SPECIES WITH MEDICINAL AND HORTICULTURAL POTENTIAL
Article number
420_19
Pages
75 – 77
Language
Abstract
A method for the regeneration of H. hemerocallidea plantlets in vitro was modified to form a continuous micropropagation system.
This was then used to establish cultures of some South African Hypoxis species with medicinal and horticultural potential, namely H. acuminata, H. rigidula, H. obtusa and H. colchicifolia. The response of each species varied with respect to growth regulation requirements, shoot multiplication and callus production.
Particular attention was paid to controlling browning of the callus and media, and internal pathogens in H. colchicifolia. The successful regeneration of plantlets of these corm forming monocotyledons in vitro required several distinct steps from inoculation to rooting.
This was then used to establish cultures of some South African Hypoxis species with medicinal and horticultural potential, namely H. acuminata, H. rigidula, H. obtusa and H. colchicifolia. The response of each species varied with respect to growth regulation requirements, shoot multiplication and callus production.
Particular attention was paid to controlling browning of the callus and media, and internal pathogens in H. colchicifolia. The successful regeneration of plantlets of these corm forming monocotyledons in vitro required several distinct steps from inoculation to rooting.
Authors
M. R. Appleton, J. van Staden
Keywords
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