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Articles

IN VITRO CULTIVATION OF NODAL SEGMENTS OF THE UMBU TREE [SPONDIAS TUBEROSA (ARR.)CAM]

Article number
447_105
Pages
535 – 538
Language
Abstract
A study dealing with in vitro cultivation of the umbu tree [Spondias tuberosa (Arr.)Cam], a Brazilian native xilophyta fruit tree was carried out to develop an appropriate micropropagation method.
Nodal segments from one-year old seedlings were collected, disinfected superficially and cultured on MS (1962) basal medium supplemented with a regime of BAP (6-benzylamino-purine) and IBA (indole-butyric-acid). After one-month cultivation period, the in vitro response was evaluated.
The best combination produced 2.2 axillary shoots/nodal segment on semi-solid culture medium containing 0.1 mg/liter BAP. It seemed that IBA and high concentration of BAP inhibited the vegetative growth.
Vitrification, callusing and polyphenol accumulation were also detected in certain concentration of the growth regulators.

Publication
Authors
N. Melo, M. Fári, J. Teixeira
Keywords
Micropropagation, axillary branching, callusing, plant growth regulators, 6-benzylamino-purine, indole-butyric-acid
Full text
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