MUTATION SPECTRUM IN DENDRANTHEMA GRANDIFLORA TZVELEV AFTER IN VIVO AND IN VITRO REGENERATION OF PLANTS FROM IRRADIATED LEAVES

Ex vivo leaf cuttings derived from plants flowering in autumn and ex vitro leaf explants derived from shoots of the plants regenerated earlier through shoot tips were used for inducing mutation in Dendranthema grandiflora cv.
Red Nero.
Leaves were irradiated with 5–25 Gy doses of X- and gamma rays.

Three vegetative mutation generations (vM₁-vM₃) of plants regenerated from irradiated leaves were observed.

The mutations obtained concerned mainly the colour of inflorescences.
The spectrum of colour changes in plants regenerated in vitro from leaf explants was greater than the spectrum in plants regenerated in vivo from leaf cuttings.
However, the range of all the mutations common for plants regenerated in vivo and in vitro was much wider than the specific range for each of the mutations.
Using gamma rays for inducing mutation was a more effective way of enlarging genetic variation spectrum in plants than using X-radiation.
The greatest mutation effect was obtained when 10–20 Gy doses of ionizing radiation were applied.

III International Symposium on In Vitro Culture and Horticultural Breeding

M. Zalewska, M. Jerzy

adventitious bud techniques, vM₁-vM₃ generation

https://doi.org/10.17660/ActaHortic.1997.447.122