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Articles

IN VITRO ANDROGENESIS IN APPLE: OPTIMIZATION OF THE ANTHER CULTURE

Article number
447_69
Pages
341 – 344
Language
Abstract
Status of haploid research in apple via anther culture and a protocol will be given.
In apple in vitro androgenesis apple, embryoid induction, regeneration rate and the ploidy level of the embryoids are mainly determined by the genotype.
By using different plant material from bud wood forced at different temperatures or from trees in the orchard, in combination with a cold pretreatment, embryoid induction frequency can remarkably improve in dependence on genotype.
After transferring the androgenic embryoids to an optimized regeneration medium, a successful regeneration has been obtained by formation of adventitious shoots.

Publication
Authors
M. Höfer
Keywords
Malus x domestica, anther culture, embryogenesis, ploidy level
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