Articles
PLANT REGENERATION FROM ANTHER-DERIVED EMBRYOS OF CABBAGE (BRASSICA OLERACEA L. VAR. CAPITATA)
This method should be efficient enough to secure plant material for creating new F1 hybrids of cabbage.
The factors taken into consideration were varieties and regeneration media.
Out of all embryos, 20,8% grew or produced shoots and 43,4% died during the first passage.
In the second passage a more intensive shoot production was observed.
On B5-2 medium, containing sucrose (20 g/l) and kinetin (20 mg/l) most embryos (18,3%) produced shoots but on MS medium containing sucrose (20 g/l), BA (1 mg/l) and NAA (0,001 mg/l), most produced callus (51,0%). The shoots rooted on B5 medium supplemented with 1 mg/l IAA. From among varieties considered in this study, most embryos of Kamienna Glowa produced shoots during first passage (16,2%) and least died (38,5%). The best medium for the regeneration was B5-2. The genotype had an effect on plant regeneration from androgenetic embryos but not as pronounced as in the process of androgenesis.
After 3 passages it was possible to obtain from each survived embryo, enough plants for the subsequent stages of cabbage breeding.