Articles
DIRECT REGENERATION OF STRAWBERRY (FRAGARIA X ANANASSA DUCH.) FROM LEAF DISKS
Article number
447_52
Pages
243 – 246
Language
Abstract
Three cultivars of strawberry (Fragaria X ananassa Duch.) were initiated in vitro from shoot tips derived from runners.
The three cultivars, ‘Camarosa’, ‘Parker’ and ‘Pajaro’ grew well in vitro on Murashige-Skoog (MS) basal salts and a modified vitamin mixture.
At 10 μM BA ‘Pajaro’ formed rosetted clumps; it elongated and grew normally when the BA concentration was reduced by half.
Both ‘Camarosa’ and Parker’ grew well and elongated satisfactorily when grown on 7.5 to 10 μM BA. Shoots were grown in culture for 6 months prior to regeneration.
Relatively high concentrations of thidiazuron (60–80 μM) were required to obtain 100% regeneration of leaf disks.
All three varieties regenerated shoots.
Small shoots appeared first after 1 month and were placed on a pre-rooting medium after 2–3 months.
One month on a low BA/IBA medium was required prior to placing the shoots on medium supplemented with 5 μM IBA in order to obtain rooting percentages close to 100%. Shoots were successfully transferred to soil and grown in the greenhouse.
These studies were carried out in preparation for transformation with pear polygalacturonase inhibiting protein.
The three cultivars, ‘Camarosa’, ‘Parker’ and ‘Pajaro’ grew well in vitro on Murashige-Skoog (MS) basal salts and a modified vitamin mixture.
At 10 μM BA ‘Pajaro’ formed rosetted clumps; it elongated and grew normally when the BA concentration was reduced by half.
Both ‘Camarosa’ and Parker’ grew well and elongated satisfactorily when grown on 7.5 to 10 μM BA. Shoots were grown in culture for 6 months prior to regeneration.
Relatively high concentrations of thidiazuron (60–80 μM) were required to obtain 100% regeneration of leaf disks.
All three varieties regenerated shoots.
Small shoots appeared first after 1 month and were placed on a pre-rooting medium after 2–3 months.
One month on a low BA/IBA medium was required prior to placing the shoots on medium supplemented with 5 μM IBA in order to obtain rooting percentages close to 100%. Shoots were successfully transferred to soil and grown in the greenhouse.
These studies were carried out in preparation for transformation with pear polygalacturonase inhibiting protein.
Authors
Ellen G. Sutter, H. Ahmadi, John M. Labavitch
Keywords
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