Articles
IN VITRO REGENERATION OF COCONUT (COCOS NUCIFERA L.) FROM IMMATURE INFLORESCENCES OF TALL CULTIVARS
Article number
447_14
Pages
121 – 122
Language
Abstract
Immature inflorescences of Tall coconut cultivars: Laguna (LAG), Bago-Oshiro (BAO) and Rennel Island Tall (RIT) were compared in terms of calloid formation and plantlet regeneration.
Inflorescence explants were excised aseptically and cultured onto semi-solid callus initiation medium with 1 x 10-4 M 2,4-D and 1 x 10-6 M 2-iP and 2.5 g/l activated charcoal.
Increasing level of 2,4-D up to 2 x 10-4 M induced calloid multiplication.
Cultivar RIT produced the most calloids.
Cultivar BAO produced the least calloids and exhibited the highest browning incidence.
The gradual reduction in the 2,4-D levels (to 3 x 10-5 M) and simultaneous increase in 2-iP levels (to 5 x 10-5 M) induced the formation of somatic embryos.
Plantlets with single and multiple shoots were regenerated from cvs.
LAG, BAO and RIT after 17 months in medium with 2-iP as cytokinin source for calloids and somatic embryos induction and BAP for embryoid maturation and germination.
The results showed that cloning the coconut is feasible.
However, the low percentage of plantlet regeneration and failure to establish plantlets ex vitro pose a limitation to large scale production of clonal coconuts via somatic embryogenesis.
Inflorescence explants were excised aseptically and cultured onto semi-solid callus initiation medium with 1 x 10-4 M 2,4-D and 1 x 10-6 M 2-iP and 2.5 g/l activated charcoal.
Increasing level of 2,4-D up to 2 x 10-4 M induced calloid multiplication.
Cultivar RIT produced the most calloids.
Cultivar BAO produced the least calloids and exhibited the highest browning incidence.
The gradual reduction in the 2,4-D levels (to 3 x 10-5 M) and simultaneous increase in 2-iP levels (to 5 x 10-5 M) induced the formation of somatic embryos.
Plantlets with single and multiple shoots were regenerated from cvs.
LAG, BAO and RIT after 17 months in medium with 2-iP as cytokinin source for calloids and somatic embryos induction and BAP for embryoid maturation and germination.
The results showed that cloning the coconut is feasible.
However, the low percentage of plantlet regeneration and failure to establish plantlets ex vitro pose a limitation to large scale production of clonal coconuts via somatic embryogenesis.
Authors
C. A. Cueto, A. W. Ebert, E. P. Rillo, O. D. Orense
Keywords
coconut, Cocos nucifera, clonal propagation, 2,4-D, 2-iP, BAP
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