CRYOPRESERVATION OF DORMANT AND NON DORMANT BROAD-LEAVED TREES

Cryopreservation is a method that makes possible the preservation of living material in liquid nitrogen, in principle indefinitely and without any physiological alteration.
The frozen material can be recovered and amplified in vitro if needed.

The methodology is of great interest for commercial in vitro propagation laboratory, for plant breeders but could also take a wide place to maintain the genetic diversity of endangered species.

AFOCEL-Biotechnology have used 2 methods to cryopreserve routinely meristems from "dormant" and non dormant buds of hardwood.
The general procedure is referred to as "fast freezing" method or "encapsulation-dehydration pre treatment" for the non dormant buds and to "slow freezing method" for "dormant" buds.
Regeneration was successfully obtained from Eucalyptus sp., Ulmus sp., Populus sp.

This paper will describe the interest and practical applications of cryopreservation regarding the conformity of the regenerated plantlets and the cost of the methodology.

III International Symposium on In Vitro Culture and Horticultural Breeding

M. Paques, M. Poissonnier, E. Dumas, V. Monod

genetic resources conservation, micropropagation, Eucalyptus sp., Ulmus sp.

https://doi.org/10.17660/ActaHortic.1997.447.99