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Articles

AGROBACTERIAL TRANSFORMATION OF ACTINIDIA KOLOMICTA

Article number
447_65
Pages
323 – 328
Language
Abstract
Efficient methods for regeneration and genetic transformation of Actinidia kolomicta has been developed.
Th highest regeneration frequency was observed on MS media with 3.0–5.0 mg/l zeatin, 0. mg/l IBA. Leaf actinidia explants were transformed by the following A. tumefaciens strains: GV3101: pBI121, A281: pPCV730, GV3101: pPCV002-CaMVC and GV3850neo.
A number of plants resistant to high concentration of kanamycin and hygromycin, and good expression of GUS gene were obtained.
The integration of NPT II gene to the plant genome was confirmed by PCR analysis.

Publication
Authors
A.P. Firsov, S.V. Dolgov
Keywords
Agrobacterium, Actinidia, transformation, kanamycin
Full text
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