MICROPROPAGATION OF ALSTROEMERIA X HYBRIDA ‘JUANITA’

Rhizome cultures of ‘Juanita’ Polish cultivar of Alstroemeria were used to develop an effective micropropagation method of the new cultivars and selections.
The effect of cytokinins (BAP=6-benzylaminopurine, kinetin=6-furfurylaminopurine, 2iP=6-[,-dimethylallylamino]purine), auxins (IAA=1H-indole-3-acetic acid, IBA=1H-indole-3-butyric acid, NAA = naphthaleneacetic acid) alone or in combination with growth retardant (paclobutrazol – ICI and flurprimidol – Dowelanco) were studied in relation to rhizome branching, aerial shoot production and rooting of rhizome.
The greatest number of aerial shoots as well as the shortest shoots were observed at the highest BAP concentration (6 mg l^-1). However, the rhizomes had the poorest rooting ability.
Application of BAP at the low concentration with paclobutrazol or flurprimidol (0.01–0.1 mg l^-1) resulted in high number of aerial shoots (5–6), reduction of their length and high rooting ability of the rhizomes.
Growth retardant applied with 1 mg l^-1 NAA strongly stimulated formation of roots and suppressed their elongation.
Low temperature treatment of the in vitro rooted rhizomes affected the early flowering of plants.

III International Symposium on In Vitro Culture and Horticultural Breeding

M. Podwyszynska, E. Gabryszewska, A. Przybyla

Alstroemeria x hybrida, micropropagation, growth retardant

https://doi.org/10.17660/ActaHortic.1997.447.32