Articles
RESULTS OF PLUM POX VIRUS TRANSMISSION INTO GERMPLASM OF PLUMS
Article number
478_44
Pages
279 – 282
Language
Abstract
In 1991 the collection of 207 plum cultivars were artificially infected by Plum Pox Virus (PPV) in permanent site.
The serotype of PPV from a tree of ‘Domestic prune’, which was not contaminated by other viruses, was used as a source of the infection. 3 buds infected by PPV were budded on one-year-old trees.
The presence of PPV was detected by ELISA. The highest increase of PPV positive cultivars was observed in the first year after infection, when PPV was transferred nearly on 70 % of trees.
The PPV was disseminated further only on the 10 % of trees during the next5 years.
Until 1996 the transmission of PPV has not been detected on 7.3 per cent of cultivars.
These are the following: ‘Bila trnecka’, ‘Dostojnaja’, ‘Francia Naranes’, ‘Iroquois’, ‘Jelta Butilcovidna’, ‘Large Sugar Prune’, ‘Reine Claude Diaphane’, ‘Renkloda Jandacek’, ‘Scoldus’, ‘Tarnina X Kirke’, ‘Valasska trnecka’, ‘Victoria’, ‘K – 4’, ‘I/14’, ‘XV/4 ŠT’. These cultivars and other negative trees were re-infected by the same source of PPV in 1996. The trees were tested by ELISA for PPV in 1997 during the time of flowering.
After both infections of PPV, there are 87.4 % of trees with positive reaction.
The PPV infection was not transferred furthermore on cultivars ‘Bila trnecka’, ‘Francia Naranes’, ‘Large Sugar Prune’, ‘Reine Claude Diaphane’, ‘Renkloda Jandacek’, ‘Scoldus’, ‘Tarnina X Kirke’, ‘Valasska trnecka’ and ‘K – 4’. The least transmission of PPV was in the trees which were contaminated by PNRSV (Prunus necrotic ringspot virus). Only local domestic cultivars ‘Bila trnecka’ and ‘Valasska trnecka’ remain free of PPV after ELISA indexing.
These cultivars are free of PDV (Prunus dwarf virus) and PNRSV.
The serotype of PPV from a tree of ‘Domestic prune’, which was not contaminated by other viruses, was used as a source of the infection. 3 buds infected by PPV were budded on one-year-old trees.
The presence of PPV was detected by ELISA. The highest increase of PPV positive cultivars was observed in the first year after infection, when PPV was transferred nearly on 70 % of trees.
The PPV was disseminated further only on the 10 % of trees during the next5 years.
Until 1996 the transmission of PPV has not been detected on 7.3 per cent of cultivars.
These are the following: ‘Bila trnecka’, ‘Dostojnaja’, ‘Francia Naranes’, ‘Iroquois’, ‘Jelta Butilcovidna’, ‘Large Sugar Prune’, ‘Reine Claude Diaphane’, ‘Renkloda Jandacek’, ‘Scoldus’, ‘Tarnina X Kirke’, ‘Valasska trnecka’, ‘Victoria’, ‘K – 4’, ‘I/14’, ‘XV/4 ŠT’. These cultivars and other negative trees were re-infected by the same source of PPV in 1996. The trees were tested by ELISA for PPV in 1997 during the time of flowering.
After both infections of PPV, there are 87.4 % of trees with positive reaction.
The PPV infection was not transferred furthermore on cultivars ‘Bila trnecka’, ‘Francia Naranes’, ‘Large Sugar Prune’, ‘Reine Claude Diaphane’, ‘Renkloda Jandacek’, ‘Scoldus’, ‘Tarnina X Kirke’, ‘Valasska trnecka’ and ‘K – 4’. The least transmission of PPV was in the trees which were contaminated by PNRSV (Prunus necrotic ringspot virus). Only local domestic cultivars ‘Bila trnecka’ and ‘Valasska trnecka’ remain free of PPV after ELISA indexing.
These cultivars are free of PDV (Prunus dwarf virus) and PNRSV.
Authors
F. Paprštein, R. Karešová, M. Navrátil
Keywords
Plum, Cultivars, Germplasm, Plum pox virus, ELISA
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