Articles
EVALUATION OF SUSCEPTIBILITY OF PISTACHIO CULTIVARS TO AFLATOXIGENIC ASPERGILLUS FLAVUS AND AFLATOXIN B1 PRODUCTION
Article number
726_113
Pages
655 – 658
Language
English
Abstract
In order to evaluate the susceptibility of pistachio cultivars to Aspergillus flavus growth and aflatoxin B1 production, ten different cultivars of pistachio, namely, Akbari, Kalleh-Ghuchi, Ohadi, Ahmadaghaei, Kalleh-Bozi, Kalkhandan, Shahpasand, FAS-13-73, Abbasali and Fakhri were collected from different parts of pistachio belt i.e.
Rafsanjan, Damghan and Ghazvin in Iran.
One isolate of aflatoxigenic Aspergillus flavus was used in this research.
This isolate of A. flavus could produce aflatoxin B1 and B2 but could not produce aflatoxin G1 and G2. In order to investigate the susceptibility of pistachio cultivars to A. flavus growth and aflatoxin B1 production, 20g lots of pistachio kernels of tested cultivars were taken in 3 replications (completely randomized design). Kernels were surface sterilized and then inoculated with 1mL spore suspension (2 × 106 spores/mL) of A. flavus. 8 days after inoculation, growth rate and colonization of A. flavus on pistachio kernels were measured.
Also, aflatoxin content of samples was extracted by BF method and estimated by TLC and densitometer.
The results of this research indicated that among different cultivars Akbari had the least susceptibility to the fungus growth rate.
The degree of aflatoxin production varied, among different cultivars.
It is observed that Kalkhandan and Fakhri had the least whereas Shahpasand and Abbasali had the greatest amount of aflatoxin B1 production.
Rafsanjan, Damghan and Ghazvin in Iran.
One isolate of aflatoxigenic Aspergillus flavus was used in this research.
This isolate of A. flavus could produce aflatoxin B1 and B2 but could not produce aflatoxin G1 and G2. In order to investigate the susceptibility of pistachio cultivars to A. flavus growth and aflatoxin B1 production, 20g lots of pistachio kernels of tested cultivars were taken in 3 replications (completely randomized design). Kernels were surface sterilized and then inoculated with 1mL spore suspension (2 × 106 spores/mL) of A. flavus. 8 days after inoculation, growth rate and colonization of A. flavus on pistachio kernels were measured.
Also, aflatoxin content of samples was extracted by BF method and estimated by TLC and densitometer.
The results of this research indicated that among different cultivars Akbari had the least susceptibility to the fungus growth rate.
The degree of aflatoxin production varied, among different cultivars.
It is observed that Kalkhandan and Fakhri had the least whereas Shahpasand and Abbasali had the greatest amount of aflatoxin B1 production.
Authors
M.M. Moghaddam, E.M. Goltapeh, H. Hokmabadi, M. Haghdel, A.M. Mortazavi
Keywords
aflatoxin G1, aflatoxin G2, TLC, densitometer
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