Articles
MOLECULAR CHARACTERIZATION OF ALMOND CULTIVARS USING MICROSATELLITE MARKERS
Article number
726_5
Pages
51 – 56
Language
English
Abstract
In recent years we have witnessed critical advances in the application of molecular markers for genetic fingerprinting in cultivated plants.
Their advantages have been widely recognized but they are even more important in woody perennials due to some particularities of these species such as their long generation time, their long individual size and their vegetative propagation.
In this work, 36 Iranian and foreign almond cultivars and 3 wild species of Amygdalus have been screened with 32 SSR primers developed in peach, to identify and characterize the genotypes and to study the genetic diversity among them.
Amplification of SSR loci was obtained for 26 of the microsatellite primer pairs and 18 of them produced polymorphic amplification patterns.
Those primer pairs produced 126 alleles with a mean value of 7 alleles per locus.
The results obtained show the usefulness of cross-species transportability of microsatellite sequences.
UPGMA cluster analysis of the similarity data grouped the genotypes studied according to their geographic origin and/or their pedigree information.
Their advantages have been widely recognized but they are even more important in woody perennials due to some particularities of these species such as their long generation time, their long individual size and their vegetative propagation.
In this work, 36 Iranian and foreign almond cultivars and 3 wild species of Amygdalus have been screened with 32 SSR primers developed in peach, to identify and characterize the genotypes and to study the genetic diversity among them.
Amplification of SSR loci was obtained for 26 of the microsatellite primer pairs and 18 of them produced polymorphic amplification patterns.
Those primer pairs produced 126 alleles with a mean value of 7 alleles per locus.
The results obtained show the usefulness of cross-species transportability of microsatellite sequences.
UPGMA cluster analysis of the similarity data grouped the genotypes studied according to their geographic origin and/or their pedigree information.
Authors
N. Amirbakhtiar, B. Shiran, H. Moradi, B.E. Sayed-Tabatabaei
Keywords
molecular markers, fingerprinting, Amygdalus, SSR, genetic diversity
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