Articles
MICROPROPAGATION OF PISTACIA MUTICA
Article number
726_28
Pages
183 – 192
Language
English
Abstract
This paper describes the micropropagation of P. mutica, which is being used as rangeland tree and rootstock for P. Vera.
P. mutica can be propagated by seed, but seed germination in the natural habitat is usually below 10%. Seeds were collected and germinated under glasshouse conditions.
Nodal segments from cuttings of glasshouse-grown plants were exposed to various tissue culture media including Murashige and Skoog, Woody Plant Medium, Alderson, B5, and a combination of these.
Significant differences between the media on shoot growth and proliferation were not observed.
Various cytokinins including BAP, Zeatin, and 2-ip were used for shoot growth and proliferation.
Finally, BAP at 20μM was the most efficient.
In vitro rooting was assessed employing various auxins including IAA, IBA and NAA. Exposure to IBA at 100μM for 5 days led to a significant improvement of root production.
Environmental conditions on rooting and weaning were also studied.
Incubation of explants in darkness did not affect root production, but shading of the basal part of explants increased rooting significantly.
Reduction of culture vessel headspace humidity from 99±1% in the conventional culture system to 90±1% in an improved culture system increased rooting significantly.
Culture in liquid medium did not result in any root production.
P. mutica can be propagated by seed, but seed germination in the natural habitat is usually below 10%. Seeds were collected and germinated under glasshouse conditions.
Nodal segments from cuttings of glasshouse-grown plants were exposed to various tissue culture media including Murashige and Skoog, Woody Plant Medium, Alderson, B5, and a combination of these.
Significant differences between the media on shoot growth and proliferation were not observed.
Various cytokinins including BAP, Zeatin, and 2-ip were used for shoot growth and proliferation.
Finally, BAP at 20μM was the most efficient.
In vitro rooting was assessed employing various auxins including IAA, IBA and NAA. Exposure to IBA at 100μM for 5 days led to a significant improvement of root production.
Environmental conditions on rooting and weaning were also studied.
Incubation of explants in darkness did not affect root production, but shading of the basal part of explants increased rooting significantly.
Reduction of culture vessel headspace humidity from 99±1% in the conventional culture system to 90±1% in an improved culture system increased rooting significantly.
Culture in liquid medium did not result in any root production.
Authors
S.R. Ghoraishi
Keywords
glasshouse, proliferation, rooting, shading, cytokinins, auxins
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