ALMOND S-GENOTYPE IDENTIFICATION BY PCR USING SPECIFIC AND NON-SPECIFIC ALLELE PRIMERS

The knowledge of the S-genotypes in the different almond cultivars has advanced quickly in the last few years, mainly by the electrophoretic separation of the stylar S-RNases as well as by the partial amplification of S-genes by PCR. The utilization of PCR primers designed from conserved sequences of almond S-RNases allows the amplification of different S-alleles, although some problems have appeared as the lack of polymorphism between the S_f and S₃ amplified PCR products when using the ConF/ConR primers.
To solve this problem, S₃ specific primers were designed to be used in the S-genotype identification of four breeding families obtained from the crosses of “Tuono” (S1Sf) by “Ferragnès” and “Ferralise” (both S₁S₃) and their reciprocals.
In addition, specific identification of the Sf allele by specific primers S_fF/S_fR was tested.

IV International Symposium on Pistachios and Almonds

J.M. Alonso, R. Socias i Company

Prunus amygdalus, breeding, marker-assisted selection.

https://doi.org/10.17660/ActaHortic.2006.726.8