ALLELE SEGREGATION AT SSR LOCI AND ALLELE TRACEABILITY FOR HAZELNUT ACCESSIONS (CORYLUS AVELLANA L.) FROM LANDRACES GROWN IN THE LATIUM REGION (ITALY)

Allele differences at six SSR loci among accessions of hazelnut landraces cultivated in the Latium region in Italy (‘Tonda Gentile Romana’, ‘Tonda di Giffoni’ and ‘Nocchione’) were determined using: (a) full-sib (FS) progenies from either controlled pairwise pollination or open pollinations of those accessions, (b) DNA purification and SSR-PCR analysis from seeds of each offspring, (c) segregation analysis of SSR-amplicons, and (d) allele annotation to the segregating amplicon database.
Except in one case, the FS parental accessions were heterozygous at each of the six SSR loci and showed independent allele segregation at any pairwise loci combination in their FS offspring.
The expected four genotypes at the six loci in the FS offspring were identified in all cases except in the FS offspring from ‘Tonda Gentile Romana’ mated to ‘Nocchione’ as male parent where at the locus CaT-B107 only one of the SSR paternal alleles was transmitted to the progeny, indicating putative linkage of the not-transmitted SSR allele with an allele causing the unilateral incompatibility when ‘Nocchione’ was used as the pollen donor.
The polymorphic PCR fragments amplified at each SSR locus favoured parentage analysis, DNA fingerprinting of the parental accessions, traceability of hazelnut seed products, detection of associations to economically important seed traits, and inference on genetic systems affecting pollen tube growth.

VII International Congress on Hazelnut

LJ. Kuzmanović, C. De Pace, E. Rugini

molecular markers, parentage analysis, DNA fingerprinting, self-incompatibility

https://doi.org/10.17660/ActaHortic.2009.845.11