Articles
GENE EXPRESSION AND SPOROPHYTIC SELF-INCOMPATIBILITY IN HAZELNUT
Article number
845_31
Pages
227 – 232
Language
English
Abstract
The European hazelnut (Corylus avellana L.) is a monoecious tree and exhibits sporophytic self-incompatibility.
Self-incompatibility is a genetic system that prevents the self-fertilization allowing the pistil to reject the pollen of genetically close individuals.
Self-incompatibility is controlled by a single multi-allelic locus, the S locus.
Sporophytic self-incompatibility (SSI) has been reported in: Asteraceae, Betulaceae, Brassicaceae, Caryophillaceae, Convolvulaceae and Sterculiaceae.
The involved molecular mechanisms are still partly unknown except for the Brassicaceae.
Studies on gene regulation of fertility, pollination and fertilization in hazelnut are very few; so with this research we propose to contribute to the knowledge about the mechanism of sporophytic self-incompatibility in hazelnut.
The Differential Display technique was applied for the study of the female determinant of self-incompatibility.
Two developmental stages of styles/stygmas were compared: before emergence from the bud and at full bloom.
The results allowed isolation of partial sequences that showed an interesting homology degree with the transmembrane serine-threonine kinase receptor of Brassica oleracea. Believing that the female determinant of self-incompatibility in hazelnut is very likely a membrane receptor, the effort in obtaining differentially expressed sequences of this type was increased.
Primers were designed on conserved regions of serine-threonine kinase receptors.
Four differentially expressed fragments were isolated from stigmas at full bloom: after blasting in TIGR and NCBI databases, one was homologous to a gene for a kinase receptor, three were homologous to kinase proteins.
The isolated sequences are being studied to check their expression in different tissues and style developmental stages.
Self-incompatibility is a genetic system that prevents the self-fertilization allowing the pistil to reject the pollen of genetically close individuals.
Self-incompatibility is controlled by a single multi-allelic locus, the S locus.
Sporophytic self-incompatibility (SSI) has been reported in: Asteraceae, Betulaceae, Brassicaceae, Caryophillaceae, Convolvulaceae and Sterculiaceae.
The involved molecular mechanisms are still partly unknown except for the Brassicaceae.
Studies on gene regulation of fertility, pollination and fertilization in hazelnut are very few; so with this research we propose to contribute to the knowledge about the mechanism of sporophytic self-incompatibility in hazelnut.
The Differential Display technique was applied for the study of the female determinant of self-incompatibility.
Two developmental stages of styles/stygmas were compared: before emergence from the bud and at full bloom.
The results allowed isolation of partial sequences that showed an interesting homology degree with the transmembrane serine-threonine kinase receptor of Brassica oleracea. Believing that the female determinant of self-incompatibility in hazelnut is very likely a membrane receptor, the effort in obtaining differentially expressed sequences of this type was increased.
Primers were designed on conserved regions of serine-threonine kinase receptors.
Four differentially expressed fragments were isolated from stigmas at full bloom: after blasting in TIGR and NCBI databases, one was homologous to a gene for a kinase receptor, three were homologous to kinase proteins.
The isolated sequences are being studied to check their expression in different tissues and style developmental stages.
Publication
Authors
D. Torello Marinoni, C. Beltramo, A. Akkak, M.L. Destefanis, P. Boccacci, R. Botta
Keywords
Corylus avellana L., S locus, female determinant, Differential Display
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