DIVERSITY IN HAZELNUT USING RAPD AND ISSR MARKERS

Corylus avellana is one of the most important economic nut species.
Over many countries several cultivars were selected from local wild populations, some of them with high local commercial value.
Fourteen Portuguese old genotypes of hazelnut and one of Turkish hazelnut, all from North Portugal, and three testers (‘Butler’, ‘Merveille’ and ‘Longue d’Espagne’ of hazelnut) were screened using 20 RAPD markers and 18 ISSR markers.
Of the 20 RAPDs primers screened 9 produced highly reproducible fragments.
These 9 primers generated 76 fragments of which 83% were polymorphic.
The ISSR markers amplified 164 fragments of which 89.7% were polymorphic.
The dendrograms generated with RAPD, ISSR and RAPD plus ISSR data, grouped the 18 genotypes in two main clusters.
The first cluster, which contains all hazelnut genotypes, can be divided into four sub-clusters.
The second cluster is formed only by the Turkish hazelnut.
It was observed that there was a good relationship between the distribution of the Portuguese genotypes in the clusters and their geographic origin.

VII International Congress on Hazelnut

S. Martins, A.P. Silva, A.A. Santos, V. Carnide

Corylus avellana, genetic diversity, molecular markers

https://doi.org/10.17660/ActaHortic.2009.845.17