A HAZELNUT BAC LIBRARY FOR MAP-BASED CLONING OF A DISEASE RESISTANCE GENE

The European hazelnut, Corylus avellana, is a diploid with a 1C genome size estimated to be 413 Mb.
Because of its small genome size, relatively short life cycle, availability of a dense linkage map, and amenability to transformation by Agrobacterium, hazelnut could serve as a model plant for the Betulaceae.
A bacterial artificial chromosome (BAC) library for selection ‘OSU 703.007’ has been constructed using the cloning enzyme MboI and the vector pECBAC1 (BamHI site). The BAC library consists of 39,936 clones arrayed in 104 384-well microtiter plates.
The average insert size is estimated to be 117 kb, with 1% of clones lacking inserts.
The genomic coverage is estimated to be about 9.5 genome-equivalents.
A map-based cloning approach is being used to isolate the ‘Gasaway’ gene which confers resistance to eastern filbert blight caused by the fungus Anisogramma anomala. OSU 703.007 is heterozygous at the resistance locus.
In order to rapidly identify specific BAC clones, we are using a PCR-based pooling and super pooling screening approach.
As a first step in chromosome walking, we are using primers designed from the sequences of RAPD markers that either flank the resistance locus or co-segregate with it.

VII International Congress on Hazelnut

V. Sathuvalli, S.A. Mehlenbacher

Corylus avellana, filbert, Anisogramma anomala, eastern filbert blight

https://doi.org/10.17660/ActaHortic.2009.845.25