IMPROVING MICROPROPAGATION OF HAZELNUT ITALIAN CULTIVARS THROUGH TEMPORARY IMMERSION SYSTEM

Temporary immersion techniques for plant in vitro culture, avoiding the time-consuming sub-culturing necessary with the use of solid media have been applied to various species in order to increase the multiplication rate and to automate as much as possible the plant production.
In this work in vitro grown shoots of Italian cultivars ‘Montebello’ and ‘Tonda Gentile Romana’ of Corylus avellana L., were cultured on a solid medium or temporary immersion system using a basal culture medium, previously defined.
Different carbon sources (sucrose or glucose) and durations of immersion (30, 60 or 120 minutes of immersion per day) were applied to determine the best cultural conditions for shoot proliferation.
The temporary immersion system enhanced shoot multiplication and gave no hyperhidric shoots.
Shoots obtained with this system were induced to root by immersion for one day in 80 mg.L^-1 IBA solution and root expression was performed either in gel rite, agar and/or vermiculite.
The most effective treatment in terms of rooting (100% and 80% in ‘Montebello’ and ‘Tonda Romana’, respectively) and acclimatisation (up to 100% in ‘Montebello’) was shown to be the combination of vermiculite with agarised medium.
Hazelnut showed a good aptitude to be in vitro multiplied with the temporary immersion system and further studies are now in progress to optimise the protocol in view of a possible wider application of temporary immersion systems (TIS) to this species.

VII International Congress on Hazelnut

E. Caboni, A. Frattarelli, M. Giorgioni, M. Meneghini, C. Damiano

acclimatisation, carbohydrates, Corylus avellana, in vitro rooting, Rita system, vermiculite

https://doi.org/10.17660/ActaHortic.2009.845.36