Articles
STRUCTURE AND GENETIC DIVERSITY OF LOCAL HAZELNUT COLLECTED IN ASTURIAS (NORTHERN SPAIN) REVEALED BY ISSR MARKERS
Article number
845_20
Pages
163 – 168
Language
English
Abstract
In the years 2003 to 2005 a hazelnut germplasm exploration was carried out in Asturias (northern Spain) and a considerable morphological diversity was found.
The present study shows the results obtained about the use of ISSR markers in the hazelnut molecular characterization and their usefulness to determinate the genetic structure of the local genetic diversity.
Fifty trees from this local germplasm survey, five local cultivars and seventeen reference and well-know cultivars were analyzed.
A total of 42 primers, containing different motifs were tested for amplifications on a panel of six cultivars.
Considering the number of fragments obtained per reaction and the number of polymorphism, 11 primers were selected to carry out this analysis.
ISSR amplification of selected primers generated multiple banding profiles in the evaluated materials with an average of 11 fragments/primer/accession and 6 polymorphism bands per primer.
These sets of primers supplied a total of 66 polymorphic bands.
A total of 24 fragments showed a significant difference between their frequency in local germplasm and their frequency in the reference cultivars.
Principal component analysis from molecular marker data was performed and variation along the two principal components accounted for 52% and 6.39% of the total variation, respectively.
The graph obtained from the two principal components grouped the accessions in two main groups corresponding to local germplasm and reference cultivars.
Cluster analysis based on Jaccard distance also showed these two main groups.
The results suggest that the local germplasm is closely related and relatively distant from germplasm of cultivars from other areas.
In consequence, the conservation of local genetic diversity of this species has high interest.
The present study shows the results obtained about the use of ISSR markers in the hazelnut molecular characterization and their usefulness to determinate the genetic structure of the local genetic diversity.
Fifty trees from this local germplasm survey, five local cultivars and seventeen reference and well-know cultivars were analyzed.
A total of 42 primers, containing different motifs were tested for amplifications on a panel of six cultivars.
Considering the number of fragments obtained per reaction and the number of polymorphism, 11 primers were selected to carry out this analysis.
ISSR amplification of selected primers generated multiple banding profiles in the evaluated materials with an average of 11 fragments/primer/accession and 6 polymorphism bands per primer.
These sets of primers supplied a total of 66 polymorphic bands.
A total of 24 fragments showed a significant difference between their frequency in local germplasm and their frequency in the reference cultivars.
Principal component analysis from molecular marker data was performed and variation along the two principal components accounted for 52% and 6.39% of the total variation, respectively.
The graph obtained from the two principal components grouped the accessions in two main groups corresponding to local germplasm and reference cultivars.
Cluster analysis based on Jaccard distance also showed these two main groups.
The results suggest that the local germplasm is closely related and relatively distant from germplasm of cultivars from other areas.
In consequence, the conservation of local genetic diversity of this species has high interest.
Publication
Authors
J.J. Ferreira, C. García, J. Tous, M. Rovira
Keywords
local germplasm exploration, Corylus avellana L., cluster analysis
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