Articles
SSR FINGERPRINTING PANEL VERIFIES IDENTITIES OF CLONES IN BACKUP HAZELNUT COLLECTION OF USDA GENEBANK
Article number
845_9
Pages
95 – 102
Language
English
Abstract
The US Department of Agriculture (USDA), Agricultural Research Service maintains a genebank representing world hazelnut (Corylus L.) diversity.
More than 670 clones are preserved as self-rooted trees in a two-hectare field planting in Corvallis, Oregon, with a single tree per accession.
In 1996 and 1997, prior to the spread of eastern filbert blight caused by Anisogramma anomala to within 75 kilometers of Corvallis, a backup collection was established in Parlier, California.
A core collection of 184 genotypes representing the wide taxonomic, geographic and phenotypic diversity of Corylus was targeted for this second planting.
Two trees of each core genotype were grafted onto seedling rootstocks over a period of five years and an orchard was established in Parlier.
The grafted trees in Parlier are at risk of identity problems due to suckers arising from below the graft union.
In May 2007, young leaves were collected from 29 Parlier trees that exhibited uncharacteristic morphological phenotypes.
A set of 12 simple sequence repeat (SSR) markers was used to fingerprint trees in the backup collection, and the results were compared to the fingerprints of the same accessions in the Corvallis collection.
Based on the results, misidentified accessions will be eliminated and the fingerprinting set will be refined further.
More than 670 clones are preserved as self-rooted trees in a two-hectare field planting in Corvallis, Oregon, with a single tree per accession.
In 1996 and 1997, prior to the spread of eastern filbert blight caused by Anisogramma anomala to within 75 kilometers of Corvallis, a backup collection was established in Parlier, California.
A core collection of 184 genotypes representing the wide taxonomic, geographic and phenotypic diversity of Corylus was targeted for this second planting.
Two trees of each core genotype were grafted onto seedling rootstocks over a period of five years and an orchard was established in Parlier.
The grafted trees in Parlier are at risk of identity problems due to suckers arising from below the graft union.
In May 2007, young leaves were collected from 29 Parlier trees that exhibited uncharacteristic morphological phenotypes.
A set of 12 simple sequence repeat (SSR) markers was used to fingerprint trees in the backup collection, and the results were compared to the fingerprints of the same accessions in the Corvallis collection.
Based on the results, misidentified accessions will be eliminated and the fingerprinting set will be refined further.
Publication
Authors
N.V. Bassil, J. Postman, K. Hummer, M. Botu, A. Sezer
Keywords
Corylus, filbert, microsatellite, germplasm, fingerprinting set
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