MOLECULAR DETECTION AND CHARACTERIZATION OF LEAF CURL VIRUS INFECTING TOMATO IN PUNJAB, INDIA

Occurrence, detection and molecular characterization of leaf curl virus infecting tomato, both under open and net house conditions were studied at the Research Farm of the Department of Vegetable Crops, Punjab Agricultural University, Ludhiana, India.
The samples were tested for the presence of begomovirus using universal primers.
Out of 42 samples studied, 20 samples were found to be positive for the begomovirus. Further, the positive samples were subjected to begomovirus identification using begomovirus specific primers, reported from India.
Among them Tomato leaf curl Palampur virus (ToLCPMV) was the most prevalent virus (18 samples) followed by Tomato leaf curl New Delhi virus (ToLCNDV) (11 samples), while, mix infection of ToLCNDV and ToLCPMV was found in a significant number of samples (9 samples). For core coat protein gene amplification, out of 20 samples, only 8 samples were able to amplify the desired product size (~870 bp). For the satellite DNA (DNA ß), only 7 samples showed desired amplification (~1.35 kb), of which, 5 samples were having mix infection of ToLCPMV and ToLCNDV. This further proves association of DNA ß with bipartite begomoviruses, causing leaf curl disease in tomato.

III International Symposium on Tomato Diseases

K.A. Gaikwad, A. Sharma, D.S. Cheema

tomato, begomovirus, molecular characterization, satellite DNA

https://doi.org/10.17660/ActaHortic.2011.914.27