Articles
EVALUATION OF THE EFFICACY OF APPLICATION OF SYSTEMIC INSECTICIDES BY RADICAL IN CONTAINING VIRAL INFECTIONS (CMV AND PVY) TRANSMITTED BY APHID VECTORS IN TOMATO
Article number
914_58
Pages
321 – 324
Language
English
Abstract
A study was performed in 2007 and 2008 by Bioagritest, in different locations in southern Italy (in Apulia and Campania regions) according to EPPO guidelines and Principles of Good Experimental Practice (GEP). Its purpose was to evaluate the efficacy of systemic insecticides applied by radical in containing viral infections transmitted by aphid vectors in tomato and induced by Cucumber mosaic virus and Potato virus Y. A local cultivar of tomato extremely susceptible to viral infections was used.
Experimental design consisted in random blocks, in 4 repetitions.
Two dosages of 3 different insecticides, active ingredients Imidacloprid, Thiamethoxam and Flonicamid, were compared with an untreated control.
The experiment consisted in 3 phases.
The first phase was performed in a thermo-conditioned greenhouse with double doors and anti-aphid nets: tomato plants were treated with two different doses of the insecticides, through absorption by radical by immersion of the polystyrene alveolate containers in a concentrated solution containing the insecticides; in the second phase plants, arranged along the edge of a canal close to a tomato field, were exposed to aphid infestation for 20, 34 and 48 days.
At the end of each period of exposure, the plants were treated with a broad-spectrum insecticide and then transferred to the laboratory.
Here (third phase) they were left 15 days in a thermo-conditioned greenhouse to allow the development of eventual viral infections and then subjected to ELISA to verify this possibility.
Evaluations have been realized about the efficacy of the tested insecticides in controlling the aphids infestation, the spread of viruses transmitted by aphids, and in the induction of any phytotoxic effects.
All the treatments, which all differed significantly from the check: the method of insecticide administration, radical prior to transplant, has shown excellent efficacy in containing both primary and secondary aphid infestations.
The insecticidal action was excellent up to a month after treatment and very effective for another 3 weeks.
The action of aphids containment showed no significant differences between the two dosages of the different formulates compared.
However, containment of viral infections carrier by aphids (CMV and PVY) showed greater efficacy when the double dosage was used.
Experimental design consisted in random blocks, in 4 repetitions.
Two dosages of 3 different insecticides, active ingredients Imidacloprid, Thiamethoxam and Flonicamid, were compared with an untreated control.
The experiment consisted in 3 phases.
The first phase was performed in a thermo-conditioned greenhouse with double doors and anti-aphid nets: tomato plants were treated with two different doses of the insecticides, through absorption by radical by immersion of the polystyrene alveolate containers in a concentrated solution containing the insecticides; in the second phase plants, arranged along the edge of a canal close to a tomato field, were exposed to aphid infestation for 20, 34 and 48 days.
At the end of each period of exposure, the plants were treated with a broad-spectrum insecticide and then transferred to the laboratory.
Here (third phase) they were left 15 days in a thermo-conditioned greenhouse to allow the development of eventual viral infections and then subjected to ELISA to verify this possibility.
Evaluations have been realized about the efficacy of the tested insecticides in controlling the aphids infestation, the spread of viruses transmitted by aphids, and in the induction of any phytotoxic effects.
All the treatments, which all differed significantly from the check: the method of insecticide administration, radical prior to transplant, has shown excellent efficacy in containing both primary and secondary aphid infestations.
The insecticidal action was excellent up to a month after treatment and very effective for another 3 weeks.
The action of aphids containment showed no significant differences between the two dosages of the different formulates compared.
However, containment of viral infections carrier by aphids (CMV and PVY) showed greater efficacy when the double dosage was used.
Publication
Authors
A. Fanigliulo, C.G. Massa , R. Pacella, A. Crescenzi
Keywords
Cucumber mosaic virus, Potato virus Y, systemic insecticides, virus control
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