IDENTIFICATION AND MANAGEMENT OF TOMATO VIRUSES IN HAWAII

A recent outbreak in hydroponically grown tomato plants showing virus-like symptoms was observed at a commercial farm on the Island of Hawaii.
Samples were collected during 2009-2010 to determine the causal agent.
The main symptoms on the tomato plants were mosaic, mottling, necrosis, chlorosis and curling of leaves with occasional stunting.
No symptoms were observed on the fruit.
Symptoms were associated with the presence of aphids.
Samples were tested for multiple viruses by ELISA and nucleic acid hybridization.
Preliminary investigations allowed us to establish that observed symptoms were associated with PVY and TEV infection.
The incidence of PVY and TEV in leaf samples was 46.4 and 50.0%, respectively.
PVY was detected in combination with TEV in over 90% of the samples tested.
Four PVY isolates were molecularly characterized by RT-PCR, cloning and sequencing of the NIb and CP gene regions.
Integrated pest management strategies for control of viral infections were implemented which helped to contribute to a decrease in disease incidence and severity in the newly transplanted hoophouses.

III International Symposium on Tomato Diseases

L. Keith, D. Gonsalves

PVY, TEV, aphid, integrated pest management (IPM)

https://doi.org/10.17660/ActaHortic.2011.914.30