Articles
TRANSMISSION AND MOLECULAR CHARACTERIZATION OF TOMATO LEAF CURL VIRUS IN ANDHRA PRADESH, SOUTH INDIA
Article number
914_38
Pages
207 – 214
Language
English
Abstract
Tomato leaf curl virus transmitted by whitefly, Bemisia tabaci is a serious viral disease of tomato causing epidemics in different states of the Indian sub-continent.
Transmission studies of ToLCV with viruliferous whitefly adults in popular tomato cultivars of Andhra Pradesh, India proved that B. tabaci successfully transmitted the ToLCV disease and a single adult whitefly can transmit the disease to an extent of 20% and maximum transmission of 100% was obtained with 15 adults/plant.
Bipartite geminivirus infecting tomatoes in Andhra Pradesh, South India were detected by polymerase chain reaction (PCR). Products derived from Rolling Circle Amplification (RCA) were cloned and sequenced.
ToLCV sample of Hyderabad, Andhra Pradesh shared a maximum identity of 93% with three Indian ToLCV strains viz., TOLCNDV-AVT1 from New Delhi (AY428769), ToLCNDV-Svr (U15015) and ToLCNDV-Chilli (EU309045). The complete nucleotide sequence of the Hyderabad sample was identified and found to be identical to Tomato leaf curl New Delhi virus (ToLCNDV) strain when it was compared with other ToLCV known sequences through BLAST analysis in the NCBI Genbank.
Transmission studies of ToLCV with viruliferous whitefly adults in popular tomato cultivars of Andhra Pradesh, India proved that B. tabaci successfully transmitted the ToLCV disease and a single adult whitefly can transmit the disease to an extent of 20% and maximum transmission of 100% was obtained with 15 adults/plant.
Bipartite geminivirus infecting tomatoes in Andhra Pradesh, South India were detected by polymerase chain reaction (PCR). Products derived from Rolling Circle Amplification (RCA) were cloned and sequenced.
ToLCV sample of Hyderabad, Andhra Pradesh shared a maximum identity of 93% with three Indian ToLCV strains viz., TOLCNDV-AVT1 from New Delhi (AY428769), ToLCNDV-Svr (U15015) and ToLCNDV-Chilli (EU309045). The complete nucleotide sequence of the Hyderabad sample was identified and found to be identical to Tomato leaf curl New Delhi virus (ToLCNDV) strain when it was compared with other ToLCV known sequences through BLAST analysis in the NCBI Genbank.
Publication
Authors
M. Rajasri, K. Vijaya lakshmi , V. Malathi , M. Sujatha, R.D.V.J. Prasada Rao
Keywords
Begomovirus, ToLCV, Tomato leaf curl virus, Bemisia tabaci transmission
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