Articles
GENETIC ANALYSIS FOR PEANUT BUD NECROSIS VIRUS (PBNV) RESISTANCE IN TOMATO (LYCOPERSICON ESCULENTUM MILL.)
Article number
914_88
Pages
459 – 463
Language
English
Abstract
Tomato (Lycopersicon esculentum Mill.) is one of the most important vegetable crops cultivated in tropical and subtropical regions of the world.
Tomato occupies 41 lakh hectares with a productivity of 26 t/ha, whereas in India it is cultivated on 5.2 lakh hectares with productivity of 14.3 t/ha.
Major reasons for low productivity are prevalence of various biotic and abiotic stresses.
Among the virus diseases, tomato tospovirus or Peanut bud necrosis virus (PBNV) transmitted by thrips has become serious in major tomato growing areas of the world including India. Tomato spotted wilt virus (TSWV) in the USA, Spain, Hawaii, Taiwan and Argentina, and peanut bud necrosis virus (PBNV) in India are the two important Tospovirus that infect tomato (Lycopersicon esculentum Mill.). Although the virus infecting tomato in India was initially reported as TSWV, it was later confirmed as PBNV. Depending on the stage of the crop and season PBNV causes yield loss up to 80-100%. Intensive cultivation of tomato has also magnified the problem.
Though several control measures viz., raising nursery under nylon net (40 mesh) and following prophylactic spray schedules against thrips have been reported PBNV cannot be controlled effectively.
Adoption of PBNV resistant cultivar/hybrid is one of the most practical and effective ways to combat with this deadly disease.
But the work on development of PBNV resistant cultivars/hybrids is very scanty.
This warrants for search of new sources of resistance to use in country breeding programme. 63 tomato entries consisting of 20 cultivars, 36 genotypes and 7 wild species were screened systematically for resistance to peanut bud necrosis virus (PBNV) under field conditions.
Only one entry, EC 5888 showed a highly resistant reaction, while EC 8630 and EC 26512 were resistant.
These five resistant lines were crossed with six high yielding, popular but susceptible varieties in a Line × Tester design.
F1, back cross and F2 populations were developed using the susceptible parents and crossed with three resistant wild species.
Segregation ratios indicated that the inheritance of resistance to PBNV fit a single dominant gene.
Direct antigen enzyme linked immunosorbent assay (DAC-ELISA) was used to distinguish healthy and infected plants.
Studies pointed out the need to exploit these crosses further to develop new breeding lines with higher level of resistance to PBNV.
Tomato occupies 41 lakh hectares with a productivity of 26 t/ha, whereas in India it is cultivated on 5.2 lakh hectares with productivity of 14.3 t/ha.
Major reasons for low productivity are prevalence of various biotic and abiotic stresses.
Among the virus diseases, tomato tospovirus or Peanut bud necrosis virus (PBNV) transmitted by thrips has become serious in major tomato growing areas of the world including India. Tomato spotted wilt virus (TSWV) in the USA, Spain, Hawaii, Taiwan and Argentina, and peanut bud necrosis virus (PBNV) in India are the two important Tospovirus that infect tomato (Lycopersicon esculentum Mill.). Although the virus infecting tomato in India was initially reported as TSWV, it was later confirmed as PBNV. Depending on the stage of the crop and season PBNV causes yield loss up to 80-100%. Intensive cultivation of tomato has also magnified the problem.
Though several control measures viz., raising nursery under nylon net (40 mesh) and following prophylactic spray schedules against thrips have been reported PBNV cannot be controlled effectively.
Adoption of PBNV resistant cultivar/hybrid is one of the most practical and effective ways to combat with this deadly disease.
But the work on development of PBNV resistant cultivars/hybrids is very scanty.
This warrants for search of new sources of resistance to use in country breeding programme. 63 tomato entries consisting of 20 cultivars, 36 genotypes and 7 wild species were screened systematically for resistance to peanut bud necrosis virus (PBNV) under field conditions.
Only one entry, EC 5888 showed a highly resistant reaction, while EC 8630 and EC 26512 were resistant.
These five resistant lines were crossed with six high yielding, popular but susceptible varieties in a Line × Tester design.
F1, back cross and F2 populations were developed using the susceptible parents and crossed with three resistant wild species.
Segregation ratios indicated that the inheritance of resistance to PBNV fit a single dominant gene.
Direct antigen enzyme linked immunosorbent assay (DAC-ELISA) was used to distinguish healthy and infected plants.
Studies pointed out the need to exploit these crosses further to develop new breeding lines with higher level of resistance to PBNV.
Publication
Authors
C. Venkata Ramana, P. Venkata Rao , R.D.V.J. Prasada Rao, S.S. Kumar, I.P. Reddy, Y.N. Reddy
Keywords
tomato, Lycopersicon esculentum, genetics of resistance, peanut bud necrosis virus (PBNV), Tomato spotted wilt virus (TSWV)
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