TRANSGENIC TOMATO PLANTS EXPRESSING PR-5 PROTEIN GENES DEMONSTRATED DISEASE RESISTANCE AGAINST PHYTOPHTHORA INFESTANS AND XANTHOMONAS VESICATORIA

Transgenic tomato plants (Solanum lycopersicum L. syn. Lycopersicon esculentum Mill. ‛Yamal’) expressed PR-5 protein genes (thau II, kiwi TLP) were obtained via Agrobacterium-mediated transformation.
The presence of the foreign genes was detected by PCR; the accumulation of the PR-5 proteins in the leaves and fruits of transgenic plants was confirmed by western blot analyses.
Seeds of T₁ and T₂ generation from transgenic plants with high expression of foreign proteins were obtained in the greenhouse.
The biological effect of the foreign PR-5 proteins was verified by greenhouse testing where T₂ transgenic tomato plants demonstrated essentially enhanced disease resistance against Phytophthora infestans and Xanthomonas vesicatoria. Homozygous T₃ generation seeds from transgenic plants with high resistance were collected.
As control of most diseases is extremely difficult, the feasibility of using PR-5 protein genes for genetic engineering plants with improved resistance to various phytopathogens will be discussed.

III International Symposium on Tomato Diseases

I.V. Korneeva , N.V. Varlamova , A.S. Pushin, A.P. Firsov , S.V. Dolgov, G.F. Monakhos, A. Motamedi Shalamzari , F.S. Dzhalilov

Agrobacterium-mediated transformation, tomato, thau II, kiwi TLP, foreign gene, protein, expression, phytopathogen

https://doi.org/10.17660/ActaHortic.2011.914.79