IN VITRO CULTURE INITIATION AND CRYOPRESERVATION OF ENDEMIC TAXA DIANTHUS GIGANTEUS SSP. BANATICUS

Dianthus giganteus D’Urv ssp. banaticus (Heuff.) Tutin (fam. Caryophylaceae) is an endemic species of the SW Carpathians, considered vulnerable/rare in Romania.
Besides the importance of these species (it is an endemic species, rare and vulnerable) its ornamental value gives it a plus for population conservation both ex situ and in situ.
Ex situ collections constitute insurance for the conservation of certain species belonging to several genotypes, from different areas.
The ex situ conservation method used in our study is cryopreservation.
The plant material was collected from natural sites.
The initiation of the in vitro cultures was done from stolons, using three sterilizing methods based on sodium hypochlorite (Domestos) and mercuric chloride.
Sterilization with sodium hypochlorite was less efficient (18-63% sterile explants), compared to the one with mercuric chloride (37-84% sterile explants), and the combined method gave the best results (60-100% sterile explants). Two combinations of plant growth regulators were used in culture media: (i) for initiation (NAA 1 mg/L; BAP 1 mg/L); (ii) for multiplication (NAA 0.1 mg/L; BAP 1 mg/L). For the cryopreservation experiments (in liquid nitrogen, at -196°C) apical meristems from in vitro-plants were used.
These meristems had 2-4 pairs of foliar primordia and a length of approximately 3-4 mm.
We also studied the influence of the treatment with different concentrations of sucrose solutions on the regeneration ability of the apices before and after cryopreservation.
The highest regeneration rate after cryopreservation was obtained with 0.5 M sucrose solution (43% regenerated apices).

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources

L. Jarda , V. Cristea, A. Halmagyi, M. Plada

https://doi.org/10.17660/ActaHortic.2011.918.18