CULTIVAR IDENTIFICATION AND GENETIC DIVERSITY ANALYSIS OF CAULIFLOWER WITH MOLECULAR MARKERS

Cauliflower is one of the most popular vegetable crops worldwide.
In this study, three molecular marker systems including RAPD, ISSR and SRAP were employed to identify cultivars and the relationship of 29 cauliflower cultivars, one broccoli and one cabbage cultivar.
The results suggested that nine RAPD primers amplified 98 distinct bands in the 32 cultivars and the polymorphic rate was 78.57%, 10 ISSR primers amplified 91 bands and the polymorphic rate was 63.74%, seven SRAP primers amplified 112 bands and the polymorphic rate was 78.57%, indicating that high polymorphism existed in 32 cultivars.
Out of all the primers, the primer me1-em4 produced 17 polymorphic bands and could distinguish 24 cultivars, while the primer me8-em8 produced 17 polymorphic bands and could distinguish 27 cultivars. 32 cultivars could be distinguished with these two primers.
Cluster analysis indicated that the genetic relationship of these cauliflower cultivars was tightly associated with its origin and characters.
It could be concluded that RAPD, ISSR and SRAP markers were powerful for cultivar identification and genetic diversity analysis in cauliflower.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources

Yan Wang, Xiaoyan Li, Jian Xu, Yuanyuan Xu, Liwang Liu

cauliflower, molecular marker, cultivar identification, genetic diversity

https://doi.org/10.17660/ActaHortic.2011.918.40