PRODUCTION AND IDENTIFICATION OF F₁ HYBRIDS BETWEEN RAPE-RADISH ADDITION LINE D AND BRASSICA OLERACEA

To transfer radish chromosome d carrying resistance against nematode Heterodera schachtii (Schmidt) to Brassica oleracea, sexual hybridization between disomic rape-radish addition line d (2n=38+2) as chromosome d donor and cabbage as recipient (2n=18) was performed.
F₁ hybrids were obtained by means of embryo rescue.
They were identified by morphological investigation, cytological and molecular marker techniques.
The results indicated that the tested F₁ plants had the same morphological characters, partly resembling female parent, partly like male parent.
The presence of chromosome d in F₁ plants was tested by the chromosome d specific RAPD marker OPF07-363 which was developed in a former project.
All F₁ plants presented the chromosome d specific band.
Furthermore, three SSR markers were used to identify the hybrid character of F₁ plants.
As a result, all investigated plants were confirmed as true hybrids.
Cytological observation of somatic cells showed that the F₁ contained 29 chromosomes.
Therefore, transfer of radish chromosome d from disomic rape-radish addition line d to cabbage was successful and the F₁ hybrids can be used as basic material for further backcrosses with cabbage.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources

Yunhua Ding, Holger Budahn, Qian Li, Lijun Li, Yuancai Jian, Jungen Kang, Li Li

rape-radish addition line, cabbage, interspecific hybrid

https://doi.org/10.17660/ActaHortic.2011.918.76