DEVELOPMENT OF IN VITRO PROPAGATION AND CONSERVATION PROTOCOLS OF THE NATIVE BRAZILIAN MANGABA TREE

The mangaba tree (Hancornia speciosa Gomes) is a native plant found in several regions of Brazil, and the fruits are used to produce pulp for juices and ice cream.
This study aimed to apply tissue culture techniques for asexual propagation and conservation of mangaba native populations from northeastern Brazil.
We studied the effect of bottle closures and explant types for development of nodal segments, and growth regulators for in vitro rhizogenesis.
For in vitro conservation, the effect of mannitol and abscisic acid in interaction with different types of explants and bottle closures was studied.
In the establishment stage, there were no significant differences for 30 or 50 days and at 65 days aluminum foil closure had the fewest shoots.
In the first and second subcultures most treatments were better than the aluminum foil closure.
There was no significant effect of explant type in the first subculture for number of adventitious shoots and number of nodal segments.
The best time for the establishment phase and the first and second subcultures was 65 days of culturing for all types of culture bottle closure.
In presence of mannitol, the shoots length was shorter than in the control, and after 90 days, a toxic effect of mannitol was observed.
Up to 90 days, the presence at 0.5 mg/L of abscisic acid presented better results for in vitro conservation of mangaba microcutting cultured in bottles sealed with aluminum foil.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources

A.S. Lédo, A.J. Sá, J.F. Silva Junior, A.V.C. Silva , L.E.C. Diniz, C.A.S. Lédo

Hancornia speciosa Gomes, genetic resources, in vitro culture, micropropagation, tissue culture

https://doi.org/10.17660/ActaHortic.2011.918.21