Articles
GENETIC IDENTITY OF APPLES, PEARS AND EUROPEAN PLUMS FROM VALDEREJO NATURAL PARK, ÁLAVA, SPAIN
Article number
918_79
Pages
631 – 638
Language
English
Abstract
Valderejo Natural Park, located in the Purón River Valley at the western corner of Alava (Basque Country, Spain) is an isolated region that became almost uninhabited around 1970, due to rural exodus.
Agriculture was practiced there since ancient times, mostly grain crops, with a certain backyard horticultural production.
Isolation and depopulation have allowed natural preservation in the valley and, in the last years, within the program of Recovery and Conservation of the Fruit Genetic Heritage kept in Valderejo Natural Park, several surveys have collected apple, pear and european plum germplasm.
The objective of this study is to determine genetic identities and potential relationships of the apple, pear and European plum prospected material with both commercial standards and ancient cultivars kept in Germplasm Banks using SSR markers.
Each accession was characterized with at least 8 highly polymorphic SSR markers that have been previously used to finger-print the commercial standards and the ancient cultivars.
For each species, the commercial standard cultivars were chosen to represent a wide genetic pool, and the ancient cultivars are the collections curated in the Germplasm Banks at Universidad Pública de Navarra (UPNA, Pamplona), Estación Experimental de Aula Dei (EEAD, Zaragoza) and Universidad de Lleida (UdL, Lleida) in Spain.
Genetic relationships among the genotypes were calculated using UPGMA and PCA analysis.
In apple and European plum, no identities with reference material were found, and PCA analysis clustered 5 apple and 8 European plum genotypes clearly apart from reference material.
Pear germplasm was closely related to the ancient cultivars Lombardia and Rosaldi. The results have proven the value of the material discovered in the park.
Agriculture was practiced there since ancient times, mostly grain crops, with a certain backyard horticultural production.
Isolation and depopulation have allowed natural preservation in the valley and, in the last years, within the program of Recovery and Conservation of the Fruit Genetic Heritage kept in Valderejo Natural Park, several surveys have collected apple, pear and european plum germplasm.
The objective of this study is to determine genetic identities and potential relationships of the apple, pear and European plum prospected material with both commercial standards and ancient cultivars kept in Germplasm Banks using SSR markers.
Each accession was characterized with at least 8 highly polymorphic SSR markers that have been previously used to finger-print the commercial standards and the ancient cultivars.
For each species, the commercial standard cultivars were chosen to represent a wide genetic pool, and the ancient cultivars are the collections curated in the Germplasm Banks at Universidad Pública de Navarra (UPNA, Pamplona), Estación Experimental de Aula Dei (EEAD, Zaragoza) and Universidad de Lleida (UdL, Lleida) in Spain.
Genetic relationships among the genotypes were calculated using UPGMA and PCA analysis.
In apple and European plum, no identities with reference material were found, and PCA analysis clustered 5 apple and 8 European plum genotypes clearly apart from reference material.
Pear germplasm was closely related to the ancient cultivars Lombardia and Rosaldi. The results have proven the value of the material discovered in the park.
Authors
M.J. Laquidáin, J. Urrestarazu, S. Crespo, L.G. Santesteban, C. Miranda , J.B. Royo
Keywords
genetic resources, fingerprint, microsatellite markers, SSR
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