BOTANICAL CHARACTERISTICS AND MOLECULAR MARKER CLASSIFICATION OF RED KWAO KRUA (BUTEA SUPERBA ROXB.)

Red Kwao Krua (Butea superba Roxb.) is a protected medicinal plant species of Thailand.
In traditional medical practices of Thailand the tuberous roots of Red Kwao Krua (RKK) are used to treat various illnesses and maintain male hormones.
Tuberous roots were obtained from forests in six provinces in northeast Thailand in the years 2004 to 2006. Molecular classification by the RAPD technique using 49 clones and 40 primers together with classification based on 10 botanical char¬acteristics was performed on the samples.
The RAPD technique and botanical classification showed that there were two groups (subgenera) at 32% relatedness. 1) Red Kwao Krua (Butea superba Roxb.) included 2 subgroups at 70% coefficient.
Subgroup 1 was RKK from Nakhon Ratchasima and Kalasin provinces.
Subgroup 2 was RKK from Nakhon Ratchasima and Sakon Nakhon provinces. 2) Tao Pun Say (Spatholobus pariviflorus (DC.) Kuntze) included 3 subgroups at 84% coefficient from Chaiyaphum, Buriram and Mahasarakham provinces.
The dendrogram of botanical characteristics also showed 2 groups at 19% relatedness and were related to the classification by DNA Markers.
Mistakes in harvesting traditional medicinal plants are very common: it may be necessary to regulate the traditional medicine industry using modern analytical methods to characterize the products.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources

Y. Manakasem , K. Muangtip, S. Sakuanrungsirikul

Butea superba Roxb., DNA markers, RAPD, Spatholobus parviflorus (DC.) Kuntze

https://doi.org/10.17660/ActaHortic.2011.918.35