GENETIC FINGERPRINTING OF THE IRISH APPLE COLLECTION USING SINGLE SEQUENCE REPEATS IN MULTIPLEX SYSTEMS – VALIDATION OF PROTOCOL

The vast majority of apples in the Irish Heritage collection are of unknown origin and have only local names.
It was therefore decided to fingerprint the collection for comparison with other databases.
Following initial attempts at using SSRs for fingerprinting the Irish apple collection, it became evident that whilst the method¬ology worked, it was of limited value unless all the major collections used the same SSRs.
Thus we decided to use a subset of 12 of the 17 SSR markers recommended by the European Cooperative Programme for Plant Genetic Resources – Fruit Group in three multiplexes of four makers each with similar requirements for PCR. This protocol was then transferred to the Agri-food and BioSciences Institute in Belfast and validated using reference material supplied by the Institut National De La Recherche Agronomique in Angers, France. ‘Bramley’s Seedling Apple’ from both the Brogdale and Loughgall collections was evaluated along with ‘Ballyvaughan Cooker’. The data demonstrated that ‘Bramley’ was identical in both collections and that ‘Ballyvaughan Cooker’ in the Irish collection was in fact ‘Bramley’.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources

S. Mac An tSaoir, F. Fernández-Fernández , C. Fleming, R. Hunter , B. Moreland

microsatellites, genetic collections

https://doi.org/10.17660/ActaHortic.2011.918.26