IN VITRO COLD-STORAGE DURATION OF CHERRY SHOOTS IS AFFECTED BY CARBON SOURCE AND NITROGEN CONCENTRATION

In vitro cold storage of fruit crop germplasm is useful for preservation of heritage or commercial cultivars.
Shoot cultures of sour cherry (Prunus cerasus L.) cultivars ‘Dolgozdannaya’, ‘Moya Radost’ and ‘Zukovskaya’, were cold stored at 4°C in either five-section tissue-culture bags or in 150-ml glass jars.
Carbon sources (3% sucrose, 2 or 3% mannitol, or 2% sucrose + 2% mannitol) were tested in Murashige and Skoog (MS) medium with or without plant growth regulators (PGRs). Nitrate nitrogen at 100, 50 or 25% of the normal MS concentration was also tested.
In some treatments, shoot cultures of the three cherry cultivars stored at 4°C remained viable for over 30 months.
A significant variation in the storage duration and significant interactions of the cultivar, treatment, and container were observed.
Sucrose was the best carbon source for all three genotypes and allowed storage for more than 30 months.
Shoots stored on 2 or 3% mannitol survived only 6 to 12 months while the combination of 2% mannitol and 2% sucrose extended storage to 30 months.
The addition of abscisic acid to 3% sucrose MS medium significantly decreased storage duration. 57 accessions of sour cherry germplasm were stored in tissue culture bags on 3% sucrose MS medium without PGRs and remained in good condition for 13 to 30 months.
Currently, 68 P. cerasus germplasm accessions of the Kazakhstan National Cherry Collection are stored in tissue-culture bags with MS medium, PGRs, and 3% sucrose.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources

I. Kovalchuk, A. Nasibulina, B.M. Reed

germplasm storage, in vitro storage, micropropagation, Prunus

https://doi.org/10.17660/ActaHortic.2011.918.20