Articles
Determination of genetic variation among melon genotypes grown in Aegean Region by morphologic and molecular markers
Article number
1297_47
Pages
347 – 358
Language
English
Abstract
Turkey, one of the secondary centers of the melon, has lots of local melon varieties.
Many of them have not been produced commercially although there is considerable variation in fruit size and shape, taste, brix and storability and disease resistance which are important for breeding programmes.
In this study, local melon varieties and commercial varieties were evaluated based on morphological and molecular markers to establish an overview of the genetic relationship among them.
Thirty-eight melon genotypes collected from various parts of Turkey by the Turkish Gene Bank and American Gene Bank were characterized by using morphological-agronomic characters and molecular markers to investigate genetic variation among them 29 morphological and agronomic characteristics such us leaf shape and color, fruit shape and weight, flesh color and firmness, brix, some features of plants according UPOV criteria and 56 polymorphic ISSR markers were used to define genetic similarity among the melon genotypes by using principal component analysis (PCA) and cluster analysis using the UPGMA in NTSYS-pc (2.2j) program.
The genetic similarity coefficient among melon genotypes based on morphological and agronomic characters was lower compared to that based on molecular markers.
This data indicated that although there is a wide morphological variation among the melon genotypes, the genetic distance among these genotypes, essentially is not far in basis of DNA markers.
Many of them have not been produced commercially although there is considerable variation in fruit size and shape, taste, brix and storability and disease resistance which are important for breeding programmes.
In this study, local melon varieties and commercial varieties were evaluated based on morphological and molecular markers to establish an overview of the genetic relationship among them.
Thirty-eight melon genotypes collected from various parts of Turkey by the Turkish Gene Bank and American Gene Bank were characterized by using morphological-agronomic characters and molecular markers to investigate genetic variation among them 29 morphological and agronomic characteristics such us leaf shape and color, fruit shape and weight, flesh color and firmness, brix, some features of plants according UPOV criteria and 56 polymorphic ISSR markers were used to define genetic similarity among the melon genotypes by using principal component analysis (PCA) and cluster analysis using the UPGMA in NTSYS-pc (2.2j) program.
The genetic similarity coefficient among melon genotypes based on morphological and agronomic characters was lower compared to that based on molecular markers.
This data indicated that although there is a wide morphological variation among the melon genotypes, the genetic distance among these genotypes, essentially is not far in basis of DNA markers.
Authors
F. Atalmış, H. Ilbi
Keywords
melon genotype, genetic diversity, morphologic marker, ISSR marker
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