Articles
Molecular characterization of Turkey’s Coruh Valley pears by SSR
Article number
1297_49
Pages
367 – 376
Language
English
Abstract
In this research it was aimed to characterize genetic relationship between 65 Coruh Valley pear genotypes and standard cultivars at molecular level.
Pear genotypes were selected from Coruh Valley biodiversity between 2011 and 2012 within the scope of the project on Investigation of Fruit Genetic Resources in Eastern Anatolian Region, which started in 1994. These genotypes preserved in the Ex situ parcel in Erzincan Horticultural Research Station.
Some international pear cultivars (Santa Maria, Coscia, B PLMO, Williams and Kieffer) and Turkish local pear cultivars (Mustafabey, Limon, Ankara and Deveci) were used as reference cultivars.
SSR method was used for molecular characterization.
SSR markers NH0021a, NH0027a, BGT23b, NH004A, KA4B and KA14 which were derived from pear genome and CH02d11, CH02b10, CH02f06 which were derived from apple genome were used because of their high polymorphism and descriptive properties.
For 74 pear genotypes (including standard cultivars) used in the study, the average expected heterozygosity value was found 0.552, the average observed heterozygosity value was found 0.425 and the mean Shannon information index value was calculated as 0.892. CH02f06 locus was the most identifying locus (PIC: 0.557, Shannon information index: 1.073). Triple alleles were observed in 2 genotypes (Arpa Pear, Boşkova Pear). The dendogram between genotypes was constructed according to UPGMA method.
In the dendogram, 6 subgroups were formed.
Two pairs of cultivars were identified to be synonyms and 4 pairs of cultivars to be homonymous.
In this research genetic relationships between Coruh Valley pear germplasm and some important standard cultivars were determined.
It is considered that the obtained data will contribute to the origin of pear cultivars from Coruh Valley, pear breeding programs and protection of genetic diversity.
Pear genotypes were selected from Coruh Valley biodiversity between 2011 and 2012 within the scope of the project on Investigation of Fruit Genetic Resources in Eastern Anatolian Region, which started in 1994. These genotypes preserved in the Ex situ parcel in Erzincan Horticultural Research Station.
Some international pear cultivars (Santa Maria, Coscia, B PLMO, Williams and Kieffer) and Turkish local pear cultivars (Mustafabey, Limon, Ankara and Deveci) were used as reference cultivars.
SSR method was used for molecular characterization.
SSR markers NH0021a, NH0027a, BGT23b, NH004A, KA4B and KA14 which were derived from pear genome and CH02d11, CH02b10, CH02f06 which were derived from apple genome were used because of their high polymorphism and descriptive properties.
For 74 pear genotypes (including standard cultivars) used in the study, the average expected heterozygosity value was found 0.552, the average observed heterozygosity value was found 0.425 and the mean Shannon information index value was calculated as 0.892. CH02f06 locus was the most identifying locus (PIC: 0.557, Shannon information index: 1.073). Triple alleles were observed in 2 genotypes (Arpa Pear, Boşkova Pear). The dendogram between genotypes was constructed according to UPGMA method.
In the dendogram, 6 subgroups were formed.
Two pairs of cultivars were identified to be synonyms and 4 pairs of cultivars to be homonymous.
In this research genetic relationships between Coruh Valley pear germplasm and some important standard cultivars were determined.
It is considered that the obtained data will contribute to the origin of pear cultivars from Coruh Valley, pear breeding programs and protection of genetic diversity.
Authors
M.R. Bozhüyük, R. Aslantaş
Keywords
pear, Coruh Valley, genetic diversity, SSR
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