Articles
miRNAs involvement on fruit development in prickly pear (Opuntia sp.)
Article number
1297_83
Pages
635 – 638
Language
English
Abstract
microRNAs (miRNAs) are small and non-coding RNA that regulate gene expression at the posttranscriptional level.
Fruit development and ripening are some of the processes regulated by miRNAs.
The miRNAs play a vital regulatory function in eukaryotic gene expression by binding specific sequences in target genes and suppressing their expression.
Little is known about miRNA on non-climacteric versus climacteric fruits development and only one previous work has been published about miRNA in Opuntia for prickly pear production.
Prickly pear development and ripening have particular features about ripening time across all different morphospecies, depending on the time required for full fruit development from anthesis to physiological maturity.
Due to its nutritional value, economic potential and characteristics to consider prickly pear a model to study, it is necessary to get appropriate integration of information about physicochemical, biochemical and molecular characteristics in this fruit, in order to understand prickly pear fruit ripening.
The aim of this work was to identify the miRNAs that participate in prickly pear fruit development.
Bioinformatics and molecular biology methods were combined to isolate, identify and select candidate miRNAs in order to analyze their expression profiles during prickly pear development. miRNA expression analysis through microarray and sRNA-seq technology for Opuntia morphospecies Robusta was performed and miRNAs expression was analyzed by RT-PCR. Here, we report 43 miRNAs and 26 targets with function in fruit development, including processes such as sexual organs development, flower induction, enhanced seed yield, biosynthesis of antioxidant and modulation of ripening through sulfate assimilation which is a critical factor for ethylene biosynthesis.
Fruit development and ripening are some of the processes regulated by miRNAs.
The miRNAs play a vital regulatory function in eukaryotic gene expression by binding specific sequences in target genes and suppressing their expression.
Little is known about miRNA on non-climacteric versus climacteric fruits development and only one previous work has been published about miRNA in Opuntia for prickly pear production.
Prickly pear development and ripening have particular features about ripening time across all different morphospecies, depending on the time required for full fruit development from anthesis to physiological maturity.
Due to its nutritional value, economic potential and characteristics to consider prickly pear a model to study, it is necessary to get appropriate integration of information about physicochemical, biochemical and molecular characteristics in this fruit, in order to understand prickly pear fruit ripening.
The aim of this work was to identify the miRNAs that participate in prickly pear fruit development.
Bioinformatics and molecular biology methods were combined to isolate, identify and select candidate miRNAs in order to analyze their expression profiles during prickly pear development. miRNA expression analysis through microarray and sRNA-seq technology for Opuntia morphospecies Robusta was performed and miRNAs expression was analyzed by RT-PCR. Here, we report 43 miRNAs and 26 targets with function in fruit development, including processes such as sexual organs development, flower induction, enhanced seed yield, biosynthesis of antioxidant and modulation of ripening through sulfate assimilation which is a critical factor for ethylene biosynthesis.
Authors
C.A. Cedillo-Jiménez, L.A. Cruz-Ramirez, C. Mondragón-Jacobo, C.A. Aguilar-Ruiz, M.A. González-Márquez, A. Arellano-Perusquia, A. Cruz-Hernández
Keywords
cactus fruit, fruit ripening, mir153, Opuntia robusta, small RNAs
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