Articles
Karyotype analysis of wild Tulipa species in China
Article number
1297_55
Pages
413 – 422
Language
English
Abstract
Chromosomal and karyotype parameters in 24 populations of eight Tulipa species, T. edulis, T. schrenkii, T. iliensis, T. thianschanica, T. altaica, T. sinkiangensis, T. heterophylla and T. buhseana, were examined using the squash technique and staining with carbol fuchsin.
Phylogenetic relationships among the 24 Tulipa populations were defined using a median linkage clustering dendrogram based on the chromosomal data.
Except for T. edulis, the karyotypes of other seven species were determined for the first time and the diploidy T. edulis (2n=2x=24) was firstly reported.
The result showed that all species observed were diploid with three chromosome types (m, sm and st). Among eight Tulipa species, the mean chromosome length was 9.48 μm and varied from 7.29 μm to 11.60 μm; the mean chromosome arm ratio was 3.11 and ranged from 2.62 to 3.70. Statistical analyses confirmed that T. altaica and T. sinkiangensis had high level of chromosome asymmetry and T. edulis had a relatively symmetry karyotype.
Cluster analysis shown that the 24 tulip populations were divided into three major groups.
Three populations of T. edulis were clustered on one branch, which had smaller chromosomes and a more symmetrical karyotype. T. altaica, T. heterophylla and T. buhseana were characterized by relatively large chromosomes and more recently developed karyotypes.
Phylogenetic relationships among the 24 Tulipa populations were defined using a median linkage clustering dendrogram based on the chromosomal data.
Except for T. edulis, the karyotypes of other seven species were determined for the first time and the diploidy T. edulis (2n=2x=24) was firstly reported.
The result showed that all species observed were diploid with three chromosome types (m, sm and st). Among eight Tulipa species, the mean chromosome length was 9.48 μm and varied from 7.29 μm to 11.60 μm; the mean chromosome arm ratio was 3.11 and ranged from 2.62 to 3.70. Statistical analyses confirmed that T. altaica and T. sinkiangensis had high level of chromosome asymmetry and T. edulis had a relatively symmetry karyotype.
Cluster analysis shown that the 24 tulip populations were divided into three major groups.
Three populations of T. edulis were clustered on one branch, which had smaller chromosomes and a more symmetrical karyotype. T. altaica, T. heterophylla and T. buhseana were characterized by relatively large chromosomes and more recently developed karyotypes.
Authors
L.W. Qu, G.M. Xing, Y.Q. Zhang, L. Xue, J.J. Chen, W. Zhang, L. Qu, J.J. Lei
Keywords
asymmetry, chromosome number, genetic relationship, karyotype, Tulipa spp
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