Articles
Ethylene suppresses anthocyanin accumulation in ‘Red Zaosu’ pear
Article number
1297_67
Pages
513 – 520
Language
English
Abstract
Plant hormones play a crucial role in the regulation of fruit color formation.
In this study, light-induced anthocyanin synthesis in postharvest fruit of ‘Red Zaosu’ pear (a hybrid of Pyrus pyrifolia Nakai and Pyrus communis L.) was inhibited by ethylene and promoted by the ethylene receptor inhibitor 1-methylcyclopropene (1-MCP), which is opposite to that in apple, strawberry and some other fruits. qPCR analysis showed that expression of most structural genes in anthocyanin biosynthesis pathway of ‘Red Zaosu’, such as PpCHS, PpCHI, PpF3H, PpDFR and PpUFGT, as well as regulatory genes PpMYB10, PpMYB114, PpbHLH33, PpbHLH3 and PpWD40 was significantly upregulated by 1-MCP treatment, while downregulated by ethephon treatment.
Furthermore, the transcription pattern of all the ERFs in pear, important transcription factors in ethylene signaling pathway, was analyzed.
A total of 21 ERFs were identified to be correlated with anthocyanin content, of which 15 ERFs negatively responded to ethylene, while 6 ERFs with EAR motif positively responded to ethylene.
However, the functions of these ERFs need to be further studied.
In this study, light-induced anthocyanin synthesis in postharvest fruit of ‘Red Zaosu’ pear (a hybrid of Pyrus pyrifolia Nakai and Pyrus communis L.) was inhibited by ethylene and promoted by the ethylene receptor inhibitor 1-methylcyclopropene (1-MCP), which is opposite to that in apple, strawberry and some other fruits. qPCR analysis showed that expression of most structural genes in anthocyanin biosynthesis pathway of ‘Red Zaosu’, such as PpCHS, PpCHI, PpF3H, PpDFR and PpUFGT, as well as regulatory genes PpMYB10, PpMYB114, PpbHLH33, PpbHLH3 and PpWD40 was significantly upregulated by 1-MCP treatment, while downregulated by ethephon treatment.
Furthermore, the transcription pattern of all the ERFs in pear, important transcription factors in ethylene signaling pathway, was analyzed.
A total of 21 ERFs were identified to be correlated with anthocyanin content, of which 15 ERFs negatively responded to ethylene, while 6 ERFs with EAR motif positively responded to ethylene.
However, the functions of these ERFs need to be further studied.
Authors
J.B. Ni, S.L. Bai, Y. Zhao, R.Y. Tao, Y.W. Teng
Keywords
anthocyanin, ethylene, 1-MCP, ERF, pear
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