Articles
Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources
Article number
1297_58
Pages
441 – 448
Language
English
Abstract
Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history.
Sweet cherries are self-incompatible determined by a gametophytic self-incompatibility system (GSI), controlled by a multi-allelic S-locus.
Maintenance and conservation of genetic resources are necessary for future breeding programs, growers and fruit production stability.
In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of genetic variability and S-genotyping within individual accessions.
In our work, we used PCR primer combinations for 20 SSR, 2 EST-SSR and 10 universal or specific S-locus markers for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy.
In total, 115 polymorphic markers were amplified by SSR markers which we used for hierarchical cluster analysis of genetic variability.
Clustering corresponded as with genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.
We confirmed previous S-genotyping for 72 accessions excepted Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Alfa, Huldra, Rivan, Vanda and Winkler’s Frühe.
It can be due to mislabelling or mistakes in previous analysis.
Totally, we detected S-locus combinations in 19 incompatibility group.
The most frequent incompatibility groups were III (S3S4), II (S1S3), IV (S2S3), and VI (S3S6).
Sweet cherries are self-incompatible determined by a gametophytic self-incompatibility system (GSI), controlled by a multi-allelic S-locus.
Maintenance and conservation of genetic resources are necessary for future breeding programs, growers and fruit production stability.
In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of genetic variability and S-genotyping within individual accessions.
In our work, we used PCR primer combinations for 20 SSR, 2 EST-SSR and 10 universal or specific S-locus markers for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy.
In total, 115 polymorphic markers were amplified by SSR markers which we used for hierarchical cluster analysis of genetic variability.
Clustering corresponded as with genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.
We confirmed previous S-genotyping for 72 accessions excepted Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Alfa, Huldra, Rivan, Vanda and Winkler’s Frühe.
It can be due to mislabelling or mistakes in previous analysis.
Totally, we detected S-locus combinations in 19 incompatibility group.
The most frequent incompatibility groups were III (S3S4), II (S1S3), IV (S2S3), and VI (S3S6).
Authors
J. Patzak, A. Henychová, F. Paprštein, J. Sedlák
Keywords
SSR and EST-SSR molecular markers, genetic diversity, cultivars, hierarchical cluster analysis, self-incompatibility, S-RNase alleles, incompatibility groups
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