Articles
Use of different LED light spectra for in vitro growth of Gardenia jasminoides Ellis ‘Grandiflora’
Article number
1417_24
Pages
191 – 198
Language
English
Abstract
LED lamps have been used in commercial in vitro propagation labs for various plant species as an alternative solution to conventional lighting systems (white, fluorescent lamps). This study aimed to evaluate the influence of blue, red, and white LED lamps compared to white, fluorescent lamps on in vitro shoot formation of Gardenia jasminoides ‘Grandiflora’ cultures.
Shoot tips (1.0-1.5 cm long) were excised from in vitro cultures and transferred to MS medium, supplemented with 20 g L‑1 sucrose and 1.13 mg L‑1 BAP and solidified with 7 g L‑1 agar.
After six weeks of culture, the shoots grown under fluorescent and blue LED light performed at best with 3.9 and 4.0 new shoots per explant, respectively, and shoot height of more than 4.8 cm.
The impact of culture conditions was evaluated through measurements of photosynthetic rate, chlorophyll fluorescence (Fv/Fm), and chlorophyll content.
Shoots grown under white fluorescent and blue LED lighting showed significantly higher photosynthetic rates (6.57 and 6.31 μmol m‑2 s‑1, respectively) compared to white and red LED lighting (4.18 and 3.75 μmol m‑2 s‑1, respectively). Similar results were observed for the chlorophyll fluorescence (Fv/Fm), where the white fluorescent and blue LED lighting (0.729 and 0.741, respectively) generated higher rates compared to white and red LED lighting (0.664 and 0.675, respectively). However, for the chlorophyll content (CCI), no significant differences were found among the four lighting treatments.
The results showed that blue LED lamps could be used cost-efficiently in gardenia in vitro culture.
Shoot tips (1.0-1.5 cm long) were excised from in vitro cultures and transferred to MS medium, supplemented with 20 g L‑1 sucrose and 1.13 mg L‑1 BAP and solidified with 7 g L‑1 agar.
After six weeks of culture, the shoots grown under fluorescent and blue LED light performed at best with 3.9 and 4.0 new shoots per explant, respectively, and shoot height of more than 4.8 cm.
The impact of culture conditions was evaluated through measurements of photosynthetic rate, chlorophyll fluorescence (Fv/Fm), and chlorophyll content.
Shoots grown under white fluorescent and blue LED lighting showed significantly higher photosynthetic rates (6.57 and 6.31 μmol m‑2 s‑1, respectively) compared to white and red LED lighting (4.18 and 3.75 μmol m‑2 s‑1, respectively). Similar results were observed for the chlorophyll fluorescence (Fv/Fm), where the white fluorescent and blue LED lighting (0.729 and 0.741, respectively) generated higher rates compared to white and red LED lighting (0.664 and 0.675, respectively). However, for the chlorophyll content (CCI), no significant differences were found among the four lighting treatments.
The results showed that blue LED lamps could be used cost-efficiently in gardenia in vitro culture.
Authors
S. Kostas, A. Economou, K. Bertsouklis, F. Bantis, N. Tsalouchos, T. Labis, S. Hatzilazarou
Keywords
chlorophyll, chlorophyll fluorescence, photosynthetic rate, shoot multiplication, tissue culture
Groups involved
- Division Vine and Berry Fruits
- Division Plant-Environment Interactions in Field Systems
- Division Horticulture for Human Health
- Working Group Vaccinium Species and Management
- Working Group Rubus and Ribes Species and Management
- Working Group Kiwifruit Culture and Management
- Working Group Strawberry Culture and Management
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